Comprehensive and quantitative stability study of ascorbic acid using capillary zone electrophoresis with ultraviolet detection and high-resolution tandem mass spectrometry
| العنوان: | Comprehensive and quantitative stability study of ascorbic acid using capillary zone electrophoresis with ultraviolet detection and high-resolution tandem mass spectrometry |
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| المؤلفون: | Sophie Goulay-Dufaÿ, Nathalie Mignet, Rabah Gahoual, Balthazar Toussaint, Caroline Roques, Pauline Legrand, Pascal Houzé, Raphaël Benattar |
| المساهمون: | Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Institut de Chimie du CNRS (INC)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Agence Générale des Equipements et Produits de Santé [Paris] (AGEPS) |
| المصدر: | Journal of Separation Science Journal of Separation Science, Wiley-VCH Verlag, 2020, 43 (14), pp.2925-2935. ⟨10.1002/jssc.202000389⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Antioxidant, medicine.medical_treatment, Filtration and Separation, Ascorbic Acid, 02 engineering and technology, Mass spectrometry, Tandem mass spectrometry, 01 natural sciences, DEGRADATION-PRODUCTS, VALIDATION, Analytical Chemistry, chemistry.chemical_compound, Capillary electrophoresis, ANTIOXIDANTS, Tandem Mass Spectrometry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, FRUITS, CZE, Chromatography, VITAMIN-C, 010401 analytical chemistry, Electrophoresis, Capillary, QUANTIFICATION, 021001 nanoscience & nanotechnology, Ascorbic acid, HYDROXYLATION, 0104 chemical sciences, Electropherogram, Electrophoresis, chemistry, ACETYLSALICYLIC-ACID, SEPARATION, Dehydroascorbic acid, 0210 nano-technology |
| الوصف: | International audience; Ascorbic acid is a powerful antioxidant compound involved in many biological functions, and a chronic deficiency is at the origin of scurvy disease. A simple, rapid, and cost-effective capillary electrophoresis method was developed for the separation and simultaneous quantification of ascorbic acid and the major degradation products: dehydroascorbic acid, furfural, and furoic acid. Systematic optimization of the conditions was performed that enabled baseline separation of the compounds in less than 10 min. In addition to simultaneous quantification of ascorbic acid alongside to the degradation products, stability studies demonstrated the possibility using capillary electrophoresis to separate and identify the major degradation products. Thus, high-resolution tandem mass spectrometry experiments were conducted in order to identify an unknown degradation product separated by capillary electrophoresis and significantly present in degraded samples. Comparison of mass spectrometry data and capillary electrophoresis electropherograms allowed to identify unambiguously trihydroxy-keto-valeraldehyde. Finally, capillary electrophoresis was successfully applied to evaluate the composition of different pharmaceutical preparation of ascorbic acid. Results showed the excellent performance of the capillary electrophoresis method due to the separation of excipients from the compounds of interest, which demonstrated the relevance of using an electrophoretic separation in order to perform comprehensive stability studies of ascorbic acid. |
| اللغة: | English |
| تدمد: | 1615-9306 1615-9314 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad80c716d872409b0625169b6f9f8f94 https://hal-cnrs.archives-ouvertes.fr/hal-03289975 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ad80c716d872409b0625169b6f9f8f94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16159306 16159314 |
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