Cytotoxic effect of endodontic irrigants in vitro
العنوان: | Cytotoxic effect of endodontic irrigants in vitro |
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المؤلفون: | Sevda Muftuoglu, Donika Bajrami, Veton Hoxha, Selen Küçükkaya, Ömer Görduysus, Nacije Dilara Zeybek |
المصدر: | Medical Science Monitor Basic Research |
سنة النشر: | 2014 |
مصطلحات موضوعية: | medicine.medical_specialty, Serial dilution, Cell Survival, Periodontal Ligament, Dentistry, Endodontics, Root canal irrigant, medicine, Periodontal fiber, Cytotoxic T cell, Animals, Viability assay, Sodium Hypochlorite - toxicity, Cytotoxicity, Cell Death, Root Canal Irrigants, business.industry, Chemistry, General Medicine, Molecular biology, In vitro, Rats, Chlorhexidine - toxicity, business, In Vitro Studies |
الوصف: | Background Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 µl/mL, using WST-1 colorimetric method. Material and methods Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. Results At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. Conclusions It seems that irrigating solutions should be used at lower concentrations to enhance cell viability. |
تدمد: | 2325-4416 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adc0e651c47d01c5da0268a073d84577 https://pubmed.ncbi.nlm.nih.gov/24614571 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....adc0e651c47d01c5da0268a073d84577 |
قاعدة البيانات: | OpenAIRE |
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