Constructs of biotin mimetic peptide with CC49 single-chain Fv designed for tumor pretargeting
العنوان: | Constructs of biotin mimetic peptide with CC49 single-chain Fv designed for tumor pretargeting |
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المؤلفون: | Gabriela Pavlinkova, David Colcher, Janina Baranowska-Kortylewicz, Surinder K. Batra, Barbara J.M. Booth |
المصدر: | Peptides. 24:353-362 |
بيانات النشر: | Elsevier BV, 2003. |
سنة النشر: | 2003 |
مصطلحات موضوعية: | Streptavidin, Time Factors, Antibodies, Neoplasm, Physiology, Dimer, Biotin, Single-Chain Fv, Biochemistry, Substrate Specificity, Mice, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Endocrinology, Antigens, Neoplasm, Iodine Isotopes, Neoplasms, Animals, Tissue Distribution, Pretargeted Radioimmunotherapy, Immunoglobulin Fragments, Chromatography, High Pressure Liquid, Pretargeting, Molecular Mimicry, Radioimmunotherapy, Molecular biology, In vitro, chemistry, Electrophoresis, Polyacrylamide Gel, Peptide mimetic, Neoplasm Transplantation |
الوصف: | Single-chain Fv constructs comprising a biotin mimetic peptide (BMP) and scFv of CC49 monoclonal antibody were produced to improve pretargeted radioimmunotherapy. BMP units that bind streptavidin were added to the carboxyl terminus of the CC49 VH region. An engineered scFvBMP monomer and a sc(Fv)2BMP dimer showed an excellent antigen recognition in vitro with a specific binding of 72±5 and 81±4 %, respectively. Properties of 125 I -sc(Fv)2BMP in mice bearing LS-174T xenografts were comparable to these of the parent 125 I -sc(Fv)2. Complexing of scFvBMPs with streptavidin increased tumor targeting and gave exceptionally high tumor-to-blood values of 63±7 for 125 I -sc(Fv)2BMP–streptavidin compared with 37±4 for sc(Fv)2BMP at 72 h after administration. High tumor and negligible normal tissue levels of these novel pretargeting constructs indicate a great potential for pretargeted radioimmunotherapy. |
تدمد: | 0196-9781 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adea8168fc9611c04082d380fad68dcd https://doi.org/10.1016/s0196-9781(03)00049-4 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....adea8168fc9611c04082d380fad68dcd |
قاعدة البيانات: | OpenAIRE |
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