R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)
| العنوان: | R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes) |
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| المؤلفون: | Tapas Charkraborty, Linyan Zhou, Sipra Mohapatra, Xiangguo Yu, Yoshitaka Nagahama, Deshou Wang, Limin Wu, Gang Liu |
| المصدر: | BMC Developmental Biology BMC Developmental Biology, Vol 12, Iss 1, p 36 (2012) |
| بيانات النشر: | BioMed Central, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Rspo1, 2, 3, Male, Expression profiles, Somatic cell, Cellular differentiation, Oryzias, In situ hybridization, Biology, Sequence Analysis, Protein, Testis, medicine, Animals, Amino Acid Sequence, RSPO1, Ovarian differentiation, lcsh:QH301-705.5, Wnt Signaling Pathway, Phylogeny, beta Catenin, Genetics, Estradiol, Ovary, Wnt signaling pathway, Molecular cloning, Brain, Gene Expression Regulation, Developmental, Cell Differentiation, Sex Determination Processes, biology.organism_classification, Cell biology, Wnt Proteins, medicine.anatomical_structure, Germ Cells, lcsh:Biology (General), Intercellular Signaling Peptides and Proteins, Female, Thrombospondins, Developmental biology, Germ cell, Developmental Biology, Research Article |
| الوصف: | Background In mammals, R-spondin (Rspo), an activator of the Wnt/β-catenin signaling pathway, has been shown to be involved in ovarian differentiation. However, the role of the Rspo/Wnt/β-catenin signaling pathway in fish gonads is still unknown. Results In the present study, full-length cDNAs of Rspo1, 2 and 3 were cloned from the gonads of medaka (Oryzias latipes). The deduced amino acid sequences of mRspo1-3 were shown to have a similar structural organization. Phylogenetic analysis showed that Rspo1, 2 and 3 were specifically clustered into three distinct clads. Tissue distribution revealed that three Rspo genes were abundantly expressed in the brain and ovary. Real-time PCR analysis around hatching (S33-5dah) demonstrated that three Rspo genes were specifically enhanced in female gonads from S38. In situ hybridization (ISH) analysis demonstrated that three Rspo genes were expressed in the germ cell in ovary, but not in testis. Fluorescence multi-color ISH showed that Rspo1 was expressed in both somatic cells and germ cells at 10dah. Exposure to ethinylestradiol (EE2) in XY individuals for one week dramatically enhanced the expression of three Rspo genes both at 0dah and in adulthood. Conclusions These results suggest that the Rspo-activating signaling pathway is involved in the ovarian differentiation and maintenance in medaka. |
| اللغة: | English |
| تدمد: | 1471-213X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afc8389b9f48b3d249233a632abae299 http://europepmc.org/articles/PMC3542121 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....afc8389b9f48b3d249233a632abae299 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1471213X |
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