An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs
| العنوان: | An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs |
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| المؤلفون: | Yanjun Yang, Youzhi Sun, Chunyuan Song, Boyue Yang, Yiping Zhao, Lianhui Wang |
| المصدر: | Nanoscale. 8:17365-17373 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Lung Neoplasms, Materials science, Early cancer, Nanotechnology, Biosensing Techniques, 02 engineering and technology, Spectrum Analysis, Raman, 010402 general chemistry, 01 natural sciences, Sensor array, Molecular beacon, microRNA, medicine, Humans, General Materials Science, Detection limit, Nanotubes, Multiple cancer, Reproducibility of Results, Cancer, 021001 nanoscience & nanotechnology, medicine.disease, 0104 chemical sciences, MicroRNAs, Clinical diagnosis, 0210 nano-technology |
| الوصف: | Simultaneous detection of multiple trace cancer associated serum miRNA biomarkers is considered as a feasible method for early cancer screening and diagnosis. In the present work, an ultrasensitive SERS sensor was prepared based on an Ag nanorod array SERS substrate by assembling special hairpin-shaped molecular beacons (MBs) for the detection of multiple lung cancer-related miRNA biomarkers. The portable SERS sensor exhibits excellent performance for the qualitative and quantitative detection of miRNAs, with advantages of ultra-sensitivity, good specificity, uniformity, reproducibility and stability, as well as remarkable reusability. By monitoring the SERS signal quenching of the MBs in the presence of target miRNA biomarkers, three lung cancer related-miRNAs (miRNA-21, miRNA-486, and miRNA-375) in buffer and human serum were simultaneously assayed using the SERS sensor array, and the limits of detection of the three miRNAs in human serum are 393 aM, 176 aM, and 144 aM, respectively. The reliable results demonstrate that the proposed SERS sensor array can be a promising candidate with great potential for the screening and clinical diagnosis of cancer in the early stage. |
| تدمد: | 2040-3372 2040-3364 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b027172f135ac1113a5b6940dc885662 https://doi.org/10.1039/c6nr05504d |
| رقم الأكسشن: | edsair.doi.dedup.....b027172f135ac1113a5b6940dc885662 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20403372 20403364 |
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