Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis
| العنوان: | Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis |
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| المؤلفون: | Cody Schmitt, James M. Tour, Redwan Huq, Teresina Laragione, Christine Beeton, Mark R. Tanner, Lizanne G. Nilewski, William K.A. Sikkema, Pércio S. Gulko, Arielle Raugh, Nejla Yosef, Carlos Perfecto Flores-Suarez |
| المصدر: | Antioxidants Volume 9 Issue 10 Antioxidants, Vol 9, Iss 1005, p 1005 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Fibroblast-like synoviocyte, Physiology, Clinical Biochemistry, Arthritis, macromolecular substances, Pharmacology, medicine.disease_cause, Biochemistry, Article, Pathogenesis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine, Rheumatoid factor, oxidative stress, Molecular Biology, nanomaterials, 030203 arthritis & rheumatology, chemistry.chemical_classification, Reactive oxygen species, Superoxide, lcsh:RM1-950, Cell Biology, medicine.disease, digestive system diseases, 030104 developmental biology, lcsh:Therapeutics. Pharmacology, chemistry, Rheumatoid arthritis, synovial fibroblast, Oxidative stress |
| الوصف: | Reactive oxygen species have been involved in the pathogenesis of rheumatoid arthritis (RA). Our goal was to determine the effects of selectively scavenging superoxide (O2&bull &minus ) and hydroxyl radicals with antioxidant nanoparticles, called poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), on the pathogenic functions of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) and on the progression of an animal model of RA. We used human FLS from patients with RA to determine PEG-HCC internalization and effects on FLS cytotoxicity, invasiveness, proliferation, and production of proteases. We used the pristane-induced arthritis (PIA) rat model of RA to assess the benefits of PEG-HCCs on reducing disease severity. PEG-HCCs were internalized by RA-FLS, reduced their intracellular O2&bull and reduced multiple measures of their pathogenicity in vitro, including proliferation and invasion. In PIA, PEG-HCCs caused a 65% reduction in disease severity, as measured by a standardized scoring system of paw inflammation and caused a significant reduction in bone and tissue damage, and circulating rheumatoid factor. PEG-HCCs did not induce lymphopenia during PIA. Our study demonstrated a role for O2&bull and hydroxyl radicals in the pathogenesis of a rat model of RA and showed efficacy of PEG-HCCs in treating a rat model of RA. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-3921 |
| DOI: | 10.3390/antiox9101005 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b16018c80affae5413599a8a3a69b246 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b16018c80affae5413599a8a3a69b246 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763921 |
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| DOI: | 10.3390/antiox9101005 |