Detection of COX-2 in liquid biopsy in patients with breast cancer
| العنوان: | Detection of COX-2 in liquid biopsy in patients with breast cancer |
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| المؤلفون: | Camilla Paulla de Souza, Jaques Waisberg, Flávia de Sousa Gehrke, Fernando Luiz Affonso Fonseca, Alípio Carmo, Beatriz da Costa Aguiar Alves |
| المصدر: | Journal of Clinical Pathology. 73:826-829 |
| بيانات النشر: | BMJ, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, Oncology, medicine.medical_specialty, Angiogenesis, Breast Neoplasms, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Internal medicine, Biomarkers, Tumor, Humans, Medicine, Outpatient clinic, Liquid biopsy, Aged, Aged, 80 and over, business.industry, Liquid Biopsy, Cancer, RNA, General Medicine, Middle Aged, medicine.disease, Reverse transcriptase, 030104 developmental biology, Cyclooxygenase 2, 030220 oncology & carcinogenesis, Female, business, Extracellular RNA |
| الوصف: | AimsTo determine the expression of the cyclooxygenase-2 (COX-2) gene in patients with breast cancer attended at the Centro Universitário Saúde ABC/Faculdade de Medicina do ABC (CUS-ABC/FMABC) outpatient clinic. Breast cancer is the most common cancer in women worldwide. More than two million new cases are reported annually. An overexpression ofCOX-2has been observed in many cancers.COX-2is related to parameters of cancer aggressiveness, including tumour size, positive nodal state and lower survival, and to angiogenesis and resistance to apoptosis.Methods15 mL of peripheral blood was obtained from 34 patients and 21 healthy women. The extracellular RNA of QIAamp RNA was submitted to an RNA sequestration kit for RNA reverse transcriptase. Quantitative real-time PCR was performed usingCOX-2-specific oligonucleotides and the endogenousGlyceraldehyde-3-Phosphate Dehydrogenasegene.ResultsThe mean remission time was 53 years. The mean progression time was 33 months. The difference observed between the patient and control groups in medianCOX-2expression (pConclusionsPatients with breast cancer showed a higher meanCOX-2expression in peripheral blood samples at diagnosis than the control group. Since this information could prove important in the diagnosis and prognosis of breast cancer, further research is required on larger patient samples. |
| تدمد: | 1472-4146 0021-9746 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b25f6583fdd574ff02d4cf21710810bc https://doi.org/10.1136/jclinpath-2020-206576 |
| رقم الأكسشن: | edsair.doi.dedup.....b25f6583fdd574ff02d4cf21710810bc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14724146 00219746 |
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