Regression of viral pathogen indicators due to improvement of hygiene protocols on boots in a bovine farm
العنوان: | Regression of viral pathogen indicators due to improvement of hygiene protocols on boots in a bovine farm |
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المؤلفون: | Satoru Takahashi, Dany Shoham, Miyuki Komura, Mizuki Ono, Makiko Yamaguchi, Amirul Hasan, Kazuaki Takehara, Chisato Daio, Mariko Ito, Humayun Kabir, Yu Miyaoka, Shahin Alam |
المصدر: | The Journal of Veterinary Medical Science |
بيانات النشر: | Japanese Society of Veterinary Science, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Veterinary medicine, Farms, media_common.quotation_subject, Cattle Diseases, Biology, exchange of boots, medicine.disease_cause, Bovine torovirus, Feces, RNA Virus Infections, Japan, Hygiene, Virology, Rotavirus, medicine, Animals, Animal Husbandry, footbath, Pathogen, viral pathogen indicators for bovine, Positive-Strand RNA Viruses, Bovine coronavirus, media_common, Full Paper, General Veterinary, biosecurity enhancement, Shoes, Virus detection, multiplex RT-PCR, Cattle, Multiplex Polymerase Chain Reaction, Bovine enterovirus |
الوصف: | Rotavirus A (RVA), bovine torovirus (BToV), bovine enterovirus (BEV) and bovine coronavirus (BCV) at a bovine farm in Ibaraki prefecture were monitored by one-step multiplex reverse transcription polymerase chain reaction (RT-PCR), with the aim of confirming the reduction of “viral pathogen indicators”. A total of 960 bovine fecal samples were collected from calves less than 2 month-old within the period from October 2016 to October 2018 every 2 months at the bovine farm. In each sampling, 40 samples were taken from calves 3 week-old or less, and 40 samples from calves over 3 week-old, in principle. At the end of September 2017, the farm introduced improvement of hygiene protocols on boots by exchanging boots and appropriate usage of a footbath at the entrance of calf sheds. In the comparison of the virus detection by RT-PCR, prevalence of all 4 viruses was significantly reduced (P |
تدمد: | 1347-7439 0916-7250 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2831b69316e79b0e58a0bb324f39f91 https://doi.org/10.1292/jvms.20-0358 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....b2831b69316e79b0e58a0bb324f39f91 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 13477439 09167250 |
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