Induced Deactivation of Genes Encoding Chlorophyll Biosynthesis Enzymes Disentangles Tetrapyrrole-Mediated Retrograde Signaling
| العنوان: | Induced Deactivation of Genes Encoding Chlorophyll Biosynthesis Enzymes Disentangles Tetrapyrrole-Mediated Retrograde Signaling |
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| المؤلفون: | Andreas Richter, Christine Glässer, Annabel Salinas Hartwig, Klaus Maier, Hagen Schlicke, Vivien Firtzlaff, Iris Finkemeier, Bernhard Grimm |
| المصدر: | Molecular Plant Mol. Plant 7, 1211-1227 (2014) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Chlorophyll, Time Factors, Nuclear gene, Arabidopsis, Protoporphyrins, Plant Science, Biology, Genes, Plant, Cyclase, chemistry.chemical_compound, Gene expression, Gene silencing, Magnesium, Gene Silencing, RNA, Messenger, Molecular Biology, Gene, Cell Nucleus, Tetrapyrrole, Chloroplast, Phenotype, Tetrapyrroles, Biochemistry, chemistry, Mg Protoporphyrin, Plastid Signal, Retrograde Signal, Tetrapyrrole Metabolism, Transcription Analysis, Seedlings, Retrograde signaling, RNA Interference, Transcriptome, Signal Transduction |
| الوصف: | In photosynthetic organisms, tetrapyrrole-mediated retrograde signals are proposed to contribute to a balanced nuclear gene expression (NGE) in response to metabolic activity in chloroplasts. We followed an experimental short-term approach that allowed the assessment of modified NGE during the first hours of specifically modified enzymatic steps of the Mg branch of tetrapyrrole biosynthesis, when pleiotropic effects of other signals can be avoided. In response to 24-h-induced silencing of CHLH , CHLM , and CHL27 encoding the CHLH subunit of Mg chelatase, the Mg protoporphyrin methyltransferase and Mg protoporphyrin monomethylester cyclase, respectively, deactivated gene expression rapidly led to reduced activity of the corresponding enzymes and altered Mg porphyrin levels. But NGE was not substantially altered. When these three genes were continuously inactivated for up to 4 d, changes of transcript levels of nuclear genes were determined. CHL27 silencing for more than 24h results in necrotic leaf lesions and modulated transcript levels of oxidative stress-responsive and photosynthesis-associated nuclear genes (PhANGs). The prolonged deactivation of CHLH and CHLM results in slightly elevated transcript levels of PhANGs and tetrapyrrole-associated genes. These time-resolved studies indicate a complex scenario for the contribution of tetrapyrrole biosynthesis on NGE mediated by 1 O 2 -induced signaling and feedback-regulated ALA synthesis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b28f07640c0c21f5ced53600099f4d9f https://hdl.handle.net/11858/00-001M-0000-0024-042A-D21.11116/0000-0005-96FA-8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b28f07640c0c21f5ced53600099f4d9f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |