Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids
| العنوان: | Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids |
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| المؤلفون: | Konstantina Alexaki, Afaf H. El-Sagheer, Antonios G. Kanaras, Tom Brown, Maria-Eleni Kyriazi, Stuart A. Lanham, Miguel Xavier, Richard O.C. Oreffo, Elloise Matthews |
| المصدر: | ACS Nano. 15:6909-6916 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | endocrine system, Stromal cell, Cell, Population, Metal Nanoparticles, General Physics and Astronomy, Adipose tissue, Bone Marrow Cells, 02 engineering and technology, Biology, 010402 general chemistry, 01 natural sciences, 03 medical and health sciences, Bone Marrow, Nucleic Acids, Bone cell, medicine, Humans, General Materials Science, education, Cells, Cultured, 030304 developmental biology, 0303 health sciences, education.field_of_study, Stem Cells, Cartilage, General Engineering, Cell Differentiation, 021001 nanoscience & nanotechnology, Chondrogenesis, 0104 chemical sciences, Cell biology, medicine.anatomical_structure, Spherical nucleic acid, Gold, Stem cell, 0210 nano-technology |
| الوصف: | Human bone marrow (BM) derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic and chondrogenic lineages enabling their application to clinical therapies. However, current methods, to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers, alone, can isolate a homogenous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.TABLE OF CONTENTS |
| وصف الملف: | text |
| تدمد: | 1936-086X 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4d76f439f582eef2f4a2941765ddf14 https://doi.org/10.1021/acsnano.0c10683 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b4d76f439f582eef2f4a2941765ddf14 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 1936086X 19360851 |
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