Biodegradation of carbazole byRalstoniasp. RJGII.123 isolated from a hydrocarbon contaminated soil
| العنوان: | Biodegradation of carbazole byRalstoniasp. RJGII.123 isolated from a hydrocarbon contaminated soil |
|---|---|
| المؤلفون: | Weiling Xue, David Warshawsky, Robert J. Grosser, Joanne Schneider, Brian K. Kinkle, Koka Jayasimhulu |
| المصدر: | Canadian Journal of Microbiology. 46:269-277 |
| بيانات النشر: | Canadian Science Publishing, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Stereochemistry, Molecular Sequence Data, Immunology, Carbazoles, Hydrocarbons, Cyclic, DNA, Ribosomal, Applied Microbiology and Biotechnology, Microbiology, High-performance liquid chromatography, Gas Chromatography-Mass Spectrometry, chemistry.chemical_compound, Bioremediation, Ralstonia, RNA, Ribosomal, 16S, Genetics, Soil Pollutants, Molecular Biology, Chromatography, High Pressure Liquid, Phylogeny, Soil Microbiology, chemistry.chemical_classification, biology, Carbazole, Betaproteobacteria, General Medicine, Biodegradation, biology.organism_classification, Soil contamination, Biodegradation, Environmental, Hydrocarbon, chemistry, Gas chromatography, Nuclear chemistry |
| الوصف: | The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole, a three-ring N-heterocyclic pollutant. Experiments were carried out with strain RJGII.123 and14C-carbazole (2 mg/L and 500 mg/L) as the sole organic carbon source. At 15 days, 80% of the 2 mg/L carbazole was recovered as CO2, and 2and ~70% remained as undegraded carbazole. Several stable intermediates were formed during this time. These intermediates were separated by high performance liquid chromatography (HPLC) and were characterized using high resolution mass spectroscopy (HR-MS) and gas chromatography - mass spectroscopy (GC-MS). At least 10 ring cleavage products of carbazole degradation were identified; four of these were confirmed as anthranilic acid, indole-2-carboxylic acid, indole-3-carboxylic acid, and (1H)-4-quinolinone by comparison with standards. These data indicate that strain RJGII.123 shares aspects of carbazole degradation with previously described Pseudomonas spp., and may be useful in facilitating the bioremediation of NHA from contaminated soils.Key words: mineralization, biodegradation, N-heterocyclic aromatic hydrocarbons, carbazole. |
| تدمد: | 1480-3275 0008-4166 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8367aeeedaad70dcbba11d728cfe3db https://doi.org/10.1139/w99-131 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b8367aeeedaad70dcbba11d728cfe3db |
| قاعدة البيانات: | OpenAIRE |
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