Polar actomyosin contractility destabilizes the position of the cytokinetic furrow
| العنوان: | Polar actomyosin contractility destabilizes the position of the cytokinetic furrow |
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| المؤلفون: | Maté Biro, Annelie Oswald, Guillaume Salbreux, Jakub Sedzinski, Ewa K. Paluch, Jean-Yves Tinevez |
| المساهمون: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Max-Planck-Gesellschaft, International Institute of Molecular and Cell Biology [Warsaw] (IIMCB), Max Planck Institute for the Physics of Complex Systems (MPI-PKS), This work was supported by the Polish Ministry of Science and Higher Education and by the Max Planck Society., We thank J. S. Bois, A. G. Clark, S. W. Grill, C. P. Heisenberg, J. Howard, A. A. Hyman, J. F. Joanny, D. K. Lubensky, A. Oates, M. Piel, I. M. Tolic-Norrelykke, W. Zachariae and M. Zerial for discussions and comments on the manuscript, and J. Roensch and the Max Planck Institute of Molecular Cell Biology and Genetics Light Microscopy Facility for technical assistance. |
| المصدر: | Nature Nature, Nature Publishing Group, 2011, 476 (7361), pp.462-466. ⟨10.1038/nature10286⟩ |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Cell division, Pyridines, [SDV]Life Sciences [q-bio], Cell, Biophysics, Nanotechnology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Cleavage (embryo), Models, Biological, Instability, Cell Line, Contractility, MESH: Cytokinesis / drug effects, MESH: Cytokinesis / physiology, MESH: Aneuploidy, medicine, Humans, Cleavage furrow, Cell Shape, Mitosis, Cell Size, Cytokinesis, MESH: Humans, Multidisciplinary, MESH: Cell Shape / drug effects, MESH: Amides / pharmacology, MESH: Models, Biological, Contractile proteins, Actomyosin, Aneuploidy, Amides, MESH: Cell Line, MESH: Cell Size / drug effects, MESH: Cell Shape / physiology, medicine.anatomical_structure, MESH: HeLa Cells, MESH: Actomyosin / metabolism, MESH: Pyridines / pharmacology, HeLa Cells |
| الوصف: | Studies of the mechanism of cytokinesis, the process by which a mother cell undergoes cleavage to form two separated daughter cells, often focus on the action of the contractile actomyosin ring at the cell equator. Ewa Paluch and colleagues instead investigate the mechanics of the actomyosin cortex found at the cell poles during cytokinesis. They find that the presence of a contractile polar cortex makes cytokinesis an inherently unstable process that can result in misalignment of the constriction ring. They propose that the membrane blebs forming at the poles of dividing cells stabilize the position by releasing cortical contractility. These findings reveal an inherent instability in the shape of a dividing cell and demonstrate a novel mechanism that helps to limit shape instability. Cytokinesis, the physical separation of daughter cells at the end of mitosis, requires precise regulation of the mechanical properties of the cell periphery1,2. Although studies of cytokinetic mechanics mostly focus on the equatorial constriction ring3, a contractile actomyosin cortex is also present at the poles of dividing cells2,4. Whether polar forces influence cytokinetic cell shape and furrow positioning remains an open question. Here we demonstrate that the polar cortex makes cytokinesis inherently unstable. We show that limited asymmetric polar contractions occur during cytokinesis, and that perturbing the polar cortex leads to cell shape oscillations, resulting in furrow displacement and aneuploidy. A theoretical model based on a competition between cortex turnover and contraction dynamics accurately accounts for the oscillations. We further propose that membrane blebs, which commonly form at the poles of dividing cells5 and whose role in cytokinesis has long been enigmatic, stabilize cell shape by acting as valves releasing cortical contractility. Our findings reveal an inherent instability in the shape of the dividing cell and unveil a novel, spindle-independent mechanism ensuring the stability of cleavage furrow positioning. |
| تدمد: | 1476-4687 0028-0836 1476-4679 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c082b86ec2f93524d871f810b1eecb36 https://doi.org/10.1038/nature10286 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....c082b86ec2f93524d871f810b1eecb36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 14764679 |
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