Background It is known that biotechnology is present in all sectors of contemporary life. Still, it is quite common the divulgation of misconceptions about it, which leads to insecurity and prejudice by the part of society. Thus, to demystify and disseminate biotechnology as science, have been developed by students of Biotechnology from Federal University of Grande Dourados (UFGD), the extension project “Biotechnology for All”. Through expository lectures the academics seek to elucidate, clarify doubts and stimulate the interest of students of the 3rd year of high school for this science. So, This study was conducted to evaluate the perception of students of the 3rd year of high school in two schools of Dourados-MS, on topics related to Biotechnology. As a result, it was possible to assess the similarities and differences between the data collected in both institutions.