Blue Light Activated Rapamycin for Optical Control of Protein Dimerization in Cells and Zebrafish Embryos
| العنوان: | Blue Light Activated Rapamycin for Optical Control of Protein Dimerization in Cells and Zebrafish Embryos |
|---|---|
| المؤلفون: | Trevor J. Horst, Alexander Deiters, Taylor M. Courtney, Kristie Darrah, Michael Tsang |
| المصدر: | ACS Chemical Biology. 16:2434-2443 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Sirolimus, chemistry.chemical_classification, Cell signaling, Embryo, Nonmammalian, Light, Chemistry, Green Fluorescent Proteins, Proteins, General Medicine, Subcellular localization, Biochemistry, Protein subcellular localization prediction, Cell biology, FKBP, Enzyme, Animals, Humans, Molecular Medicine, Protein Dimerization, Dimerization, Zebrafish, Function (biology), HeLa Cells, Subcellular Fractions, Blue light |
| الوصف: | Rapamycin-induced dimerization of FKBP and FRB is the most commonly utilized chemically induced protein dimerization system. It has been extensively used to conditionally control protein localization, split-enzyme activity, and protein-protein interactions in general by simply fusing FKBP and FRB to proteins of interest. We have developed a new aminonitrobiphenylethyl caging group and applied it to the generation of a caged rapamycin analog that can be photoactivated using blue light. Importantly, the caged rapamycin analog shows minimal background activity with regard to protein dimerization and can be directly interfaced with a wide range of established (and often commercially available) FKBP/FRB systems. We have successfully demonstrated its applicability to the optical control of enzymatic function, protein stability, and protein subcellular localization. Further, we also showcased its applicability toward optical regulation of cell signaling, specifically mTOR signaling, in cells and aquatic embryos. |
| تدمد: | 1554-8937 1554-8929 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb1438b466a40e92aaaca9ed6d454cd0 https://doi.org/10.1021/acschembio.1c00547 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....cb1438b466a40e92aaaca9ed6d454cd0 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15548937 15548929 |
|---|