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Influence of differential expression of acetylcholinesterase in brain and muscle on respiration

التفاصيل البيبلوغرافية
العنوان: Influence of differential expression of acetylcholinesterase in brain and muscle on respiration
المؤلفون: Arthur S. Foutz, Palmer Taylor, Shelley Camp, Jean Champagnat, Eliane Boudinot, Véronique Bernard, Eric Krejci
المساهمون: Neurobiologie génétique et intégrative (NGI), Centre National de la Recherche Scientifique (CNRS), Institut de Neurobiologie Alfred Fessard (INAF), Biologie des Jonctions Neuromusculaires Normales et Pathologiques (U686), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of pharmacology, University of California [San Diego] (UC San Diego), University of California-University of California, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS)
المصدر: Respiratory Physiology and Neurobiology
Respiratory Physiology and Neurobiology, Elsevier, 2009, 165 (1), pp.40-8. ⟨10.1016/j.resp.2008.10.003⟩
Respiratory Physiology and Neurobiology, Elsevier, 2009, 165 (1), pp.40-8. 〈10.1016/j.resp.2008.10.003〉
بيانات النشر: HAL CCSD, 2009.
سنة النشر: 2009
مصطلحات موضوعية: Male, MESH: Muscles, MESH: Body Temperature, Physiology, MESH: Anoxia, MESH: Pulmonary Ventilation, MESH: Tidal Volume, MESH: Mice, Knockout, Body Temperature, Hypercapnia, Mice, chemistry.chemical_compound, 0302 clinical medicine, Respiratory function, MESH: Animals, Respiratory system, Hypoxia, Butyrylcholinesterase, MESH: Plethysmography, Whole Body, Sequence Deletion, Mice, Knockout, 0303 health sciences, MESH: Butyrylcholinesterase, Muscles, Respiration, General Neuroscience, Brain, Exons, MESH: Sequence Deletion, Acetylcholinesterase, MESH: Gene Expression Regulation, Bronchodilator Agents, medicine.anatomical_structure, Knockout mouse, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Terbutaline, Acetylcholine, medicine.drug, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Biology, Article, Neuromuscular junction, 03 medical and health sciences, MESH: Brain, Internal medicine, MESH: Analysis of Variance, Terbutaline, Tidal Volume, medicine, Animals, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Mice, Gene knockout, Plethysmography, Whole Body, 030304 developmental biology, MESH: Respiration, Analysis of Variance, MESH: Acetylcholinesterase, MESH: Male, Endocrinology, Gene Expression Regulation, chemistry, MESH: Hypercapnia, MESH: Bronchodilator Agents, Pulmonary Ventilation, MESH: Exons, MESH: Female, 030217 neurology & neurosurgery
الوصف: International audience; A mouse strain with a deleted acetylcholinesterase (AChE) gene (AChE knockout) shows a decreased inspiration time and increased tidal volume and ventilation .To investigate the respective roles of AChE in brain and muscle, we recorded respiration by means of whole-body plethysmography in knockout mice with tissue selective deletions in AChE expression. A mouse strain with the anchoring domains of AChE deleted (del E5+6 knockout mice) has very low activity in the brain and neuromuscular junction, but increased monomeric AChE in serum. A mouse strain with deletion of the muscle specific region of AChE (del i1RR knockout mice) exhibits no expression in muscle, but unaltered expression in the central nervous system. Neither strain exhibits the pronounced phenotypic traits observed in the complete AChE knockout strain. A third strain lacking the anchor molecule PRiMA, has no functional AChE and butyrylcholinesterase (BChE) in brain and an unaltered respiratory function. BChE inhibition by bambuterol decreases tidal volume and body temperature in del E5+6 and i1RR knockout strains, but not in PRiMA deletion or wild-type controls. We find that: (1) deletion of the full AChE gene is required for a pronounced alteration in respiratory phenotype, (2) BChE is involved in respiratory muscles contraction and temperature control in del E5+6 and i1RR knockout mice, and (3) AChE expression requiring a gene product splice to either exons 5 and 6 or regulated by intron1 influences temperature control.
اللغة: English
تدمد: 1569-9048
1878-1519
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0214f1870820114756efaf784d46a8c
https://hal.archives-ouvertes.fr/hal-00374370
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....d0214f1870820114756efaf784d46a8c
قاعدة البيانات: OpenAIRE
الوصف
تدمد:15699048
18781519