In situ product recovery as a powerful tool to improve the fermentative production of muconic acid in Saccharomyces cerevisiae
| العنوان: | In situ product recovery as a powerful tool to improve the fermentative production of muconic acid in Saccharomyces cerevisiae |
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| المؤلفون: | Sinah Tönjes, Evelien Uitterhaegen, Pieter De Brabander, Ellen Verhoeven, Tom Delmulle, Karel De Winter, Wim Soetaert |
| المصدر: | BIOCHEMICAL ENGINEERING JOURNAL |
| بيانات النشر: | Elsevier BV, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Agriculture and Food Sciences, Environmental Engineering, Fermentation, Biomedical Engineering, In situ product recovery, Biocompatibility, Bioengineering, Saccharomyces cerevisiae, Muconic acid, Reactive extraction, Biotechnology |
| الوصف: | Muconic acid has gained interest as a bio-based platform chemical to produce value-added compounds including adipic acid and terephthalic acid. In this study, a fermentation strategy based on in situ product recovery was established to boost process performance and counteract product inhibition. An optimized reactive extraction mixture for muconic acid was developed consisting of 12.5% (v/v) CYTOP 503 in canola oil and showing high extraction capacity as well as biocompatibility. By integrating the reactive extraction into the fermentation at shake flask level, growth and muconic acid titer could be increased by 44% and 18%, respectively. The process was successfully transferred to a fed-batch fermentation in a 10 L bioreactor with a final muconic acid titer of 4.33 g L−1 and maximum productivity of 0.053 g L−1, demonstrating the potential of this strategy to enhance challenging fermentation processes for industrial biochemicals. |
| وصف الملف: | application/pdf |
| تدمد: | 1369-703X 1873-295X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d14b13aeabf6fa71facc6f6d7507547b https://doi.org/10.1016/j.bej.2022.108746 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d14b13aeabf6fa71facc6f6d7507547b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1369703X 1873295X |
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