Metagenome Survey of a Multispecies and Alga-Associated Biofilm Revealed Key Elements of Bacterial-Algal Interactions in Photobioreactors
| العنوان: | Metagenome Survey of a Multispecies and Alga-Associated Biofilm Revealed Key Elements of Bacterial-Algal Interactions in Photobioreactors |
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| المؤلفون: | Andreas Pommerening-Röser, Adam Grundhoff, Wolfgang R. Streit, Simon Güllert, Ines Krohn-Molt, Anja Poehlein, Christel Schmeisser, Dieter Hanelt, Malik Alawi, Rolf Daniel, Bernd Wemheuer |
| المصدر: | Applied and Environmental Microbiology. 79:6196-6206 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | DNA, Bacterial, Microorganism, Molecular Sequence Data, Population, Bacterial Physiological Phenomena, DNA, Ribosomal, 7. Clean energy, Applied Microbiology and Biotechnology, Microbiology, Photobioreactors, 03 medical and health sciences, RNA, Ribosomal, 16S, Microalgae, education, Betaproteobacteria, 030304 developmental biology, 0303 health sciences, education.field_of_study, Bacteria, Ecology, biology, 030306 microbiology, Alphaproteobacteria, Biofilm, Bacteroidetes, Biodiversity, Sequence Analysis, DNA, biology.organism_classification, B vitamins, Biofilms, Microbial Interactions, Food Science, Biotechnology |
| الوصف: | Photobioreactors (PBRs) are very attractive for sunlight-driven production of biofuels and capturing of anthropogenic CO 2 . One major problem associated with PBRs however, is that the bacteria usually associated with microalgae in nonaxenic cultures can lead to biofouling and thereby affect algal productivity. Here, we report on a phylogenetic, metagenome, and functional analysis of a mixed-species bacterial biofilm associated with the microalgae Chlorella vulgaris and Scenedesmus obliquus in a PBR. The biofilm diversity and population dynamics were examined through 16S rRNA phylogeny. Overall, the diversity was rather limited, with approximately 30 bacterial species associated with the algae. The majority of the observed microorganisms were affiliated with Alphaproteobacteria , Betaproteobacteria , and Bacteroidetes . A combined approach of sequencing via GS FLX Titanium from Roche and HiSeq 2000 from Illumina resulted in the overall production of 350 Mbp of sequenced DNA, 165 Mbp of which was assembled in larger contigs with a maximum size of 0.2 Mbp. A KEGG pathway analysis suggested high metabolic diversity with respect to the use of polymers and aromatic and nonaromatic compounds. Genes associated with the biosynthesis of essential B vitamins were highly redundant and functional. Moreover, a relatively high number of predicted and functional lipase and esterase genes indicated that the alga-associated bacteria are possibly a major sink for lipids and fatty acids produced by the microalgae. This is the first metagenome study of microalga- and PBR-associated biofilm bacteria, and it gives new clues for improved biofuel production in PBRs. |
| تدمد: | 1098-5336 0099-2240 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7ec85c778d7f2c960daf93962de11aa https://doi.org/10.1128/aem.01641-13 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d7ec85c778d7f2c960daf93962de11aa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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