Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub
العنوان: | Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub |
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المؤلفون: | Aurélie Malzac, Marie Malissen, Kartiek Kanduri, Frédéric Di Fiore, Rachel Joly, Amandine Sansoni, Riitta Lahesmaa, Simon Hauri, Harri Lähdesmäki, Ruedi Aebersold, Zhi Chen, Shou Yamasaki, Bernard Malissen, Matthias Gstaiger, Takashi Saito, Romain Roncagalli, Yinming Liang |
المصدر: | Nature Immunology. 15(4):384-392 |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Antigens, Differentiation, T-Lymphocyte, CD4-Positive T-Lymphocytes, Proteomics, VAV1, Immunology, Quantitative proteomics, Receptors, Antigen, T-Cell, Mice, Transgenic, Biology, Article, Protein–protein interaction, Mice, Antigens, CD, T-Lymphocyte Subsets, Animals, Immunology and Allergy, Calcium Signaling, Proto-Oncogene Proteins c-vav, Cells, Cultured, Adaptor Proteins, Signal Transducing, ZAP-70 Protein-Tyrosine Kinase, ZAP70, T-cell receptor, ta1182, Membrane Proteins, Signal transducing adaptor protein, Phosphoproteins, Molecular biology, Cell biology, Mice, Inbred C57BL, Multiprotein Complexes, Signal transduction, Protein Binding |
الوصف: | T cell antigen receptor (TCR)-mediated activation of T cells requires the interaction of dozens of proteins. Here we used quantitative mass spectrometry and activated primary CD4(+) T cells from mice in which a tag for affinity purification was knocked into several genes to determine the composition and dynamics of multiprotein complexes that formed around the kinase Zap70 and the adaptors Lat and SLP-76. Most of the 112 high-confidence time-resolved protein interactions we observed were previously unknown. The surface receptor CD6 was able to initiate its own signaling pathway by recruiting SLP-76 and the guanine nucleotide-exchange factor Vav1 regardless of the presence of Lat. Our findings provide a more complete model of TCR signaling in which CD6 constitutes a signaling hub that contributes to the diversification of TCR signaling. |
تدمد: | 1529-2908 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc95fa53be235902c57e44841e73b1c6 https://doi.org/10.1038/ni.2843 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....dc95fa53be235902c57e44841e73b1c6 |
قاعدة البيانات: | OpenAIRE |
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