Single-Cell Analyses Revealed Transfer Ranges of IncP-1, IncP-7, and IncP-9 Plasmids in a Soil Bacterial Community
| العنوان: | Single-Cell Analyses Revealed Transfer Ranges of IncP-1, IncP-7, and IncP-9 Plasmids in a Soil Bacterial Community |
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| المؤلفون: | Masaki Shintani, Kazuhide Kimbara, Atsushi Yamazoe, Jun-ichi Inoue, Shoko Ohji, Kazuhiro Matsui, Moriya Ohkuma, Hideaki Nojiri, Akira Hosoyama |
| المصدر: | Applied and Environmental Microbiology. 80:138-145 |
| بيانات النشر: | American Society for Microbiology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | DNA, Bacterial, Gene Transfer, Horizontal, Firmicutes, Green Fluorescent Proteins, Molecular Sequence Data, DNA, Ribosomal, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Microbiology, Actinobacteria, Plasmid, Genes, Reporter, RNA, Ribosomal, 16S, Environmental Microbiology, Soil Microbiology, Genetics, Bacteria, Ecology, biology, Bacteroidetes, Sequence Analysis, DNA, Flow Cytometry, biology.organism_classification, Pseudomonas putida, Delftia, Conjugation, Genetic, Single-Cell Analysis, Soil microbiology, Plasmids, Food Science, Biotechnology |
| الوصف: | The conjugative transfer ranges of three different plasmids of the incompatibility groups IncP-1 (pBP136), IncP-7 (pCAR1), and IncP-9 (NAH7) were investigated in soil bacterial communities by culture-dependent and culture-independent methods. Pseudomonas putida , a donor of each plasmid, was mated with soil bacteria, and green fluorescent protein (GFP), encoded on the plasmid, was used as a reporter protein for successful transfer. GFP-expressing transconjugants were detected and separated at the single-cell level by flow cytometry. Each cell was then analyzed by PCR and sequencing of its 16S rRNA gene following either whole-genome amplification or cultivation. A large number of bacteria within the phylum Proteobacteria was identified as transconjugants for pBP136 by both culture-dependent and culture-independent methods. Transconjugants belonging to the phyla Actinobacteria , Bacteroidetes , and Firmicutes were detected only by the culture-independent method. Members of the genus Pseudomonas (class Gammaproteobacteria ) were identified as major transconjugants of pCAR1 and NAH7 by both methods, whereas Delftia species (class Betaproteobacteria ) were detected only by the culture-independent method. The transconjugants represented a minority of the soil bacteria. Although pCAR1-containing Delftia strains could not be cultivated after a one-to-one filter mating assay between the donor and cultivable Delftia strains as recipients, fluorescence in situ hybridization detected pCAR1-containing Delftia cells, suggesting that Delftia was a “transient” host of pCAR1. |
| تدمد: | 1098-5336 0099-2240 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde8d50a5c7f13e5239fa3ce91dee249 https://doi.org/10.1128/aem.02571-13 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dde8d50a5c7f13e5239fa3ce91dee249 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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