Rapid Detection to Differentiate Hypervirulent Klebsiella pneumoniae (hvKp) From Classical K. pneumoniae by Identifying peg-344 With Loop-Mediated Isothermal Amplication (LAMP)
| العنوان: | Rapid Detection to Differentiate Hypervirulent Klebsiella pneumoniae (hvKp) From Classical K. pneumoniae by Identifying peg-344 With Loop-Mediated Isothermal Amplication (LAMP) |
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| المؤلفون: | Xiaobing Liu, Dan-Dan Wei, Yuling Feng, Wenjian Liao, Qi-sen Huang, Yang Liu, Dan Long, La-Gen Wan, Wei Zhang |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2020) |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), loop-mediated isothermal amplication, Klebsiella pneumoniae, Loop-mediated isothermal amplification, lcsh:QR1-502, Virulence, easy, Microbiology, lcsh:Microbiology, law.invention, 03 medical and health sciences, law, PEG ratio, Methods, hypervirulent K. pneumoniae, Polymerase chain reaction, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Chemistry, K pneumoniae, peg-344, Gold standard (test), biology.organism_classification, Molecular diagnostics, rapid |
| الوصف: | Objectives To establish a rapid molecular diagnostics of hvKp using the peg-344 loop-mediated isothermal amplification technique (LAMP). Methods In all, 28 K. pneumoniae strains isolated from the blood of patients were used for the peg-344 LAMP. K. pneumoniae NTUH-K2044 and K. pneumoniae ATCC700603 were used as positive control and negative control, respectively. For comparison, all the results were detected in a polymerase chain reaction (PCR), which was considered the gold standard for the detection of the gene. Mouse lethality assay, and Serum killing assay were also used to determine the virulence phenotype of K. pneumoniae. Results We determined the specificity and sensitivity of the primers for peg-344 detection in the LAMP reactions. This LAMP assay was able to specifically differentiate hvKp from classical K. pneumoniae (cKp) at 65°C, which was 100-fold more sensitive than a PCR assay for peg-344 detection. The virulence phenotype of K. pneumoniae detected by LAMP was as precise as by Mouse lethality assay and Serum killing assay. Conclusion The LAMP assay is easy to perform and rapid. Therefore, it can be routinely applied to differentiate hvKp from cKp in the clinical laboratory. |
| اللغة: | English |
| تدمد: | 1664-302X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de15be8c2f03e6caddad4d86f820992d http://europepmc.org/articles/PMC7325879 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....de15be8c2f03e6caddad4d86f820992d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
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