Prognostic significance of GAD1 overexpression in patients with resected lung adenocarcinoma
| العنوان: | Prognostic significance of GAD1 overexpression in patients with resected lung adenocarcinoma |
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| المؤلفون: | Kiyoshi Masuda, Hiromitsu Takizawa, Kazuya Kondo, Koichiro Kajiura, Shoichiro Tange, Akira Tangoku, Tomohiro Kohmoto, Issei Imoto, Mitsuhiro Tsuboi |
| المصدر: | Cancer Medicine Cancer Medicine, Vol 8, Iss 9, Pp 4189-4199 (2019) |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Oncology, Cancer Research, medicine.medical_specialty, Lung Neoplasms, Adenocarcinoma of Lung, lcsh:RC254-282, Epigenesis, Genetic, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, expression, Biomarkers, Tumor, medicine, Humans, Radiology, Nuclear Medicine and imaging, Stage (cooking), Pathological, Aged, Neoplasm Staging, Original Research, DNA methylation, Lung, GAD1, Glutamate Decarboxylase, business.industry, Clinical Cancer Research, Sequence Analysis, DNA, Methylation, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Prognosis, lung adenocarcinoma, medicine.disease, Survival Analysis, Up-Regulation, Gene Expression Regulation, Neoplastic, Treatment Outcome, 030104 developmental biology, medicine.anatomical_structure, CpG site, 030220 oncology & carcinogenesis, Adenocarcinoma, Immunohistochemistry, Female, business |
| الوصف: | Background and Objectives In a previous genome‐wide screening, we identified hypermethylated CpG islands around glutamate decarboxylase 1 (GAD1) in lung adenocarcinoma (LADC). In this study, we aimed to investigate the methylation and expression status of GAD1 and its prognostic value in patients with LADC. Methods GAD1 methylation and mRNA expression status were analyzed using 33 tumorous and paired non‐tumorous LADC samples and publicly available datasets. The prognostic value of GAD1 overexpression was investigated using publicly available datasets of mRNA levels and 162 cases of LADC by immunohistochemistry. Results The methylation and mRNA expression levels of GAD1, each having a positive correlation, were significantly higher in LADC tumors than in paired non‐tumorous tissues. LADC patients with higher GAD1 mRNA expression showed significantly poorer prognosis for overall survival in publicly available datasets. Higher immunoreactivity of GAD1 was significantly associated with the pathological stage, pleural invasion, lymph vessel invasion, and poorer prognosis for cancer‐specific and disease‐free survival. Multivariate analysis revealed that GAD1 protein overexpression is an independent prognosticator for disease‐free survival. Conclusions GAD1 mRNA and protein expression levels were significant prognostic factors in LADC, suggesting that they might be useful biomarkers to stratify patients with worse clinical outcomes after resection. |
| تدمد: | 2045-7634 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfc5d75b459f9c5470d6d415e644d2f8 https://doi.org/10.1002/cam4.2345 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dfc5d75b459f9c5470d6d415e644d2f8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20457634 |
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