miR‐451a is downregulated and targets PSMB8 in prostate cancer
| العنوان: | miR‐451a is downregulated and targets PSMB8 in prostate cancer |
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| المؤلفون: | Huan‐Zhi Yang, Yong‐Jun Jiang, Li‐Qi Xu, Yun Liu |
| المصدر: | Kaohsiung Journal of Medical Sciences, Vol 36, Iss 7, Pp 494-500 (2020) |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Proteasome Endopeptidase Complex, Apoptosis, PSMB8, colony formation, 03 medical and health sciences, 0302 clinical medicine, Western blot, Antigens, CD, Cell Movement, Genes, Reporter, Cell Line, Tumor, microRNA, Humans, Medicine, Luciferase, Luciferases, Base Pairing, lcsh:R5-920, Reporter gene, Oligoribonucleotides, miR‐451a, Base Sequence, medicine.diagnostic_test, business.industry, Cell growth, Molecular Mimicry, Prostate, General Medicine, Cadherins, cell invasion, prostate cancer, Gene Expression Regulation, Neoplastic, MicroRNAs, cell proliferation, Cell culture, 030220 oncology & carcinogenesis, Cancer research, 030211 gastroenterology & hepatology, lcsh:Medicine (General), business, Plasmids, Signal Transduction |
| الوصف: | Abnormal expression of microRNAs (miRNAs) is frequently occurred in prostate cancer (PCa). This study was aimed to investigate the biological roles of miR‐451a in PCa. Quantitative real‐time PCR (qRT‐PCR) and Western blot were employed to investigate the expression levels of miR‐451a and proteasome (prosome, macropain) subunit, beta type, 8 (PSMB8) in PCa cell lines. Luciferase activity reporter assay was used to verify the connection between miR‐451a and PSMB8. in vitro functional experiments were performed to measure the effects of miR‐451a or PSMB8 on PCa cell proliferation, colony formation ability, cell invasion, and cell apoptosis. miR‐451a expression was downregulated, whereas PSMB8 expression was upregulated in PCa cell lines. Luciferase activity reporter assay confirmed the direct connection between miR‐451a and PSMB8. Overexpression of miR‐451a inhibits PCa cell proliferation, colony formation, cell invasion and promotes cell apoptosis, while the overexpression of PSMB8 caused the opposite effects. Moreover, rescue experiments confirmed PSMB8 was a functional target of miR‐451a. In conclusion, this study provides novel insights into the role of miR‐451a in PCa, and the results demonstrated miR‐451a could inhibit PCa progression by targeting PSMB8. |
| تدمد: | 2410-8650 1607-551X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0bceb198ce4ba062f771be91c6b0adc https://doi.org/10.1002/kjm2.12196 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e0bceb198ce4ba062f771be91c6b0adc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24108650 1607551X |
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