Effect of ambient temperature on respiratory tract cells exposed to SARS-CoV-2 viral mimicking nanospheres—An experimental study
| العنوان: | Effect of ambient temperature on respiratory tract cells exposed to SARS-CoV-2 viral mimicking nanospheres—An experimental study |
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| المؤلفون: | Sapun H. Parekh, Arsalan Nisar, Daniel S Wang, Christian M Jennings, Sayantan Chatterjee, Natasha Nehra, Alexandra Paul, Sachin Kumar, Sabine Pütz |
| المصدر: | Biointerphases |
| بيانات النشر: | American Vacuum Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | viruses, General Physics and Astronomy, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Virus, Microbiology, Biomaterials, Mice, Coated Materials, Biocompatible, Viral envelope, Cell Line, Tumor, medicine, Animals, Humans, General Materials Science, Bovine serum albumin, Receptor, Lung, Coronavirus, biology, SARS-CoV-2, Chemistry, Binding protein, Temperature, COVID-19, Epithelial Cells, Articles, General Chemistry, medicine.anatomical_structure, Cell culture, Spike Glycoprotein, Coronavirus, NIH 3T3 Cells, biology.protein, Angiotensin-Converting Enzyme 2, Nanospheres, Respiratory tract |
| الوصف: | The novel coronavirus caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has reached more than 160 countries and has been declared a pandemic. SARS-CoV-2 infects host cells by binding to the angiotensin-converting enzyme 2 (ACE-2) surface receptor via the spike (S) receptor-binding protein (RBD) on the virus envelope. Global data on a similar infectious disease spread by SARS-CoV-1 in 2002 indicated improved stability of the virus at lower temperatures facilitating its high transmission in the community during colder months (December–February). Seasonal viral transmissions are strongly modulated by temperatures, which can impact viral trafficking into host cells; however, an experimental study of temperature-dependent activity of SARS-CoV-2 is still lacking. We mimicked SARS-CoV-2 with polymer beads coated with the SARS-CoV-2 S protein to study the effect of seasonal temperatures on the binding of virus-mimicking nanospheres to lung epithelia. The presence of the S protein RBD on nanosphere surfaces led to binding by Calu-3 airway epithelial cells via the ACE-2 receptor. Calu-3 and control fibroblast cells with S-RBD-coated nanospheres were incubated at 33 and 37 °C to mimic temperature fluctuations in the host respiratory tract, and we found no temperature dependence in contrast to nonspecific binding of bovine serum ablumin-coated nanospheres. Moreover, the ambient temperature changes from 4 to 40 °C had no effect on S-RBD-ACE-2 ligand-receptor binding and minimal effect on the S-RBD protein structure (up to 40 °C), though protein denaturing occurred at 51 °C. Our results suggest that ambient temperatures from 4 to 40 °C have little effect on the SARS-CoV-2-ACE-2 interaction in agreement with the infection data currently reported. |
| تدمد: | 1559-4106 1934-8630 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e440573cc712cce7d2424932fe3aed8e https://doi.org/10.1116/6.0000743 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e440573cc712cce7d2424932fe3aed8e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Scitation. | تدمد: | 15594106 19348630 |
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