Accumulation of Decarboxylated S-Adenosyl-l-Methionine in Mammalian Cells as a Consequence of the Inhibition of Putrescine Biosynthesis
| العنوان: | Accumulation of Decarboxylated S-Adenosyl-l-Methionine in Mammalian Cells as a Consequence of the Inhibition of Putrescine Biosynthesis |
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| المؤلفون: | Mirlyse Siat, Pierre S. Mamont, Charles Danzin, Nicole Claverie, Joseph Wagner, Anne-Marie Joder-Ohlenbusch |
| المصدر: | European Journal of Biochemistry. 123:499-504 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, Ornithine, Adenosylmethionine Decarboxylase, endocrine system, Eflornithine, Carboxy-Lyases, Biology, environment and public health, Biochemistry, chemistry.chemical_compound, Liver Neoplasms, Experimental, S-Adenosyl-l-methionine, Polyamines, Putrescine, Animals, heterocyclic compounds, Cells, Cultured, organic chemicals, Prostate, Substrate (chemistry), Rats, Inbred Strains, Ornithine Decarboxylase Inhibitors, Rat hepatoma, In vitro, Rats, Spermidine, enzymes and coenzymes (carbohydrates), Putrescine biosynthesis, chemistry, Transmethylation |
| الوصف: | Biological transmethylation reactions and polyamine biosynthesis share the substrate S-adenosyl-L-methionine. Under normal conditions, decarboxylated S-adenosyl-L-methionine, the aminopropyl donor for polyamine biosynthesis, does not accumulate because of its rapid utilization in spermidine and spermine synthesis. Alteration of polyamine synthesis by DL-alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of L-ornithine decarboxylase, leads to a striking accumulation of decarboxylated S-adenosyl-L-methionine in rat hepatoma cells cultured in vitro and in rat ventral prostate. This increase is due both to lack of putrescine and spermidine for the aminopropyltransferase reactions and to the elevation of S-adenosyl-L-methionine decarboxylase activity. The biological implications of accumulation of decarboxylated S-adenosyl-L-methionine are discussed with regard to the regulation of S-adenosyl-L-methionine decarboxylase activity and to the antiproliferative effects of DL-alpha-difluoromethylornithine. |
| تدمد: | 1432-1033 0014-2956 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e87ef6a9507ebb1649917da0f7e768c7 https://doi.org/10.1111/j.1432-1033.1982.tb06559.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e87ef6a9507ebb1649917da0f7e768c7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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