Temporal Effects on Radiation Responses in Nonhuman Primates: Identification of Biofluid Small Molecule Signatures by Gas Chromatography–Mass Spectrometry Metabolomics
| العنوان: | Temporal Effects on Radiation Responses in Nonhuman Primates: Identification of Biofluid Small Molecule Signatures by Gas Chromatography–Mass Spectrometry Metabolomics |
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| المؤلفون: | Simon Authier, Kim Bujold, Albert J. Fornace, Michael Girgis, Evagelia C. Laiakis, James Bakke, Sarah E. Dowd, Janet Gahagen, Polly Y. Chang, Suraj Dhungana, Evan L. Pannkuk, Denise Nishita |
| المصدر: | Metabolites, Vol 9, Iss 5, p 98 (2019) Metabolites Volume 9 Issue 5 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, biodosimetry, nonhuman primates, Endocrinology, Diabetes and Metabolism, lcsh:QR1-502, Urine, Biochemistry, Article, lcsh:Microbiology, acute radiation syndrome, Ionizing radiation, 03 medical and health sciences, 0302 clinical medicine, Metabolomics, Biodosimetry, Metabolome, Microbiome, Molecular Biology, mass spectrometry, Chemistry, Acute Radiation Syndrome, metabolomics, 030104 developmental biology, 030220 oncology & carcinogenesis, GC-MS, Gas chromatography–mass spectrometry, ionizing radiation |
| الوصف: | Whole body exposure to ionizing radiation damages tissues leading to physical symptoms which contribute to acute radiation syndrome. Radiation biodosimetry aims to determine characteristic early biomarkers indicative of radiation exposure and is necessary for effective triage after an unanticipated radiological incident. Radiation metabolomics can address this aim by assessing metabolic perturbations following exposure. Gas chromatography&ndash mass spectrometry (GC-MS) is a standardized platform ideal for compound identification. We performed GC time-of-flight MS for the global profiling of nonhuman primate urine and serum samples up to 60 d after a single 4 Gy &gamma ray total body exposure. Multivariate statistical analysis showed higher group separation in urine vs. serum. We identified biofluid markers involved in amino acid, lipid, purine, and serotonin metabolism, some of which may indicate host microbiome dysbiosis. Sex differences were observed for amino acid fold changes in serum samples. Additionally, we explored mitochondrial dysfunction by tricarboxylic acid intermediate analysis in the first week with a GC tandem quadrupole MS platform. By adding this temporal component to our previous work exploring dose effects at 7 d, we observed the highest fold changes occurring at 3 d, returning closer to basal levels by 7 d. These results emphasize the utility of both MS-based metabolomics for biodosimetry and complementary analytical platforms for increased metabolome coverage. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-1989 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc53a84e8e4ce5b8f2292cfb31301d2 https://doi.org/10.3390/metabo9050098 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ebc53a84e8e4ce5b8f2292cfb31301d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22181989 |
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