Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva Switzerland
| العنوان: | Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva Switzerland |
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| المؤلفون: | Gesuele Renzi, Sandrine Bernabeu, Laurent Poirel, Patrice Nordmann, Jacques Schrenzel, Abdessalam Cherkaoui |
| المصدر: | Journal of Antimicrobial Chemotherapy; Vol 66 Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, Vol. 66, No 8 (2011) pp. 1730-3 |
| بيانات النشر: | Oxford University Press, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Klebsiella pneumoniae, Plasmids/analysis, Quinolones, Polymerase Chain Reaction, Hospitals, University, Plasmid, Switzerland/epidemiology, Beta-Lactam Resistance, TRNA Methyltransferases/genetics, Pharmacology (medical), Carbapenems/pharmacology, health care economics and organizations, ddc:616, 0303 health sciences, tRNA Methyltransferases, Enterobacteriaceae Infections, Enterobacteriaceae/classification/drug effects/enzymology/genetics, 3. Good health, Acinetobacter baumannii, Anti-Bacterial Agents, Infectious Diseases, Conjugation, Genetic, Switzerland, Plasmids, Microbiology (medical), DNA, Bacterial, Gene Transfer, Horizontal, Sequence analysis, macromolecular substances, Biology, beta-Lactam Resistance, beta-Lactamases, Microbiology, Anti-Bacterial Agents/pharmacology, 03 medical and health sciences, Enterobacteriaceae, DNA, Bacterial/chemistry/genetics, parasitic diseases, Humans, 030304 developmental biology, Pharmacology, 030306 microbiology, Enterobacteriaceae Infections/epidemiology/microbiology, Sequence Analysis, DNA, biology.organism_classification, Proteus mirabilis, TRNA Methyltransferases, New Delhi metallo-beta-lactamase 1, Carbapenems, biology.protein, Quinolones/pharmacology, Multilocus sequence typing, Beta-Lactamases/genetics, Multilocus Sequence Typing |
| الوصف: | Objectives To analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in Geneva University Hospitals, Switzerland. Methods PCR and sequencing were used to identify β-lactamases, 16S RNA methylases and plasmid-mediated quinolone resistance genes. The transferable properties of the plasmids were analysed, as well as their plasmid type. The strains were typed by multilocus sequence typing. Results Three patients were found to be positive for NDM-1-producing enterobacterial isolates (one with Escherichia coli and Klebsiella pneumoniae, one with K. pneumoniae only and one with Proteus mirabilis), where NDM-1 stands for New Delhi metallo-β-lactamase-1. The blaNDM-1 carbapenemase gene was detected in all isolates in addition to genes encoding narrow-spectrum β-lactamases (TEM-1, SHV-11, OXA-1, OXA-9 and OXA-10), extended-spectrum β-lactamases (CTX-M-15, CMY-16 and CMY-30), ArmA and quinolone resistance determinants (Qnr). The blaNDM-1 gene was located on conjugative IncA/C- or IncF-type plasmids. Upstream of the blaNDM-1 gene, part of ISAba125, previously identified in NDM-1-negative Acinetobacter baumannii, was found. Downstream of the blaNDM-1 gene, variable sequences were found. Conclusions This work constitutes the first identification of NDM-1 producers in Switzerland. Interestingly, patients from whom these NDM-1-producing isolates were recovered had a link with the Indian subcontinent or the Balkans |
| اللغة: | English |
| تدمد: | 0305-7453 |
| DOI: | 10.1093/jac/dkr174 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec6625507f638e1b1b35a453c2a58c5c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ec6625507f638e1b1b35a453c2a58c5c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03057453 |
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| DOI: | 10.1093/jac/dkr174 |