Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital
| العنوان: | Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital |
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| المؤلفون: | Ramy K. Aziz, Amani El-Kholy, Arwa Ramadan El-Manakhly, Noha S. Soliman, Shahira AbdelSalam ElBanna, May S. Soliman |
| المصدر: | Genes Volume 11 Issue 10 Genes, Vol 11, Iss 1219, p 1219 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Methicillin-Resistant Staphylococcus aureus, 0301 basic medicine, lcsh:QH426-470, Virulence Factors, 030106 microbiology, Leukocidin, Virulence, methicillin- resistant Staphylococcus aureus (MRSA), Biology, medicine.disease_cause, virulome, whole-genome sequencing (WGS), Article, DNA sequencing, Microbiology, Tertiary Care Centers, 03 medical and health sciences, Drug Resistance, Multiple, Bacterial, Genotype, Genetics, medicine, Humans, next-generation sequencing (NGS), resistome, Genetic Association Studies, Genetics (clinical), SCCmec, High-Throughput Nucleotide Sequencing, Genomics, Staphylococcal Infections, Methicillin-resistant Staphylococcus aureus, Anti-Bacterial Agents, Resistome, lcsh:Genetics, 030104 developmental biology, Staphylococcus aureus |
| الوصف: | Methicillin-resistant Staphylococcus aureus (MRSA) strains are associated with serious complications and poor clinical outcome. In Egypt, they contribute to more than 70% of S. aureus healthcare-associated infections. This study combined whole-genome sequencing, bioinformatics, and statistical analyses to identify the phylogeny, resistome, virulome and potential genotype&ndash phenotype&ndash clinical correlation among 18 clinical isolates of MRSA in a tertiary hospital in Cairo, Egypt. The ST1535-V MRSA clone was the most frequently isolated (16.6%), followed by ST5-VI, ST1-V and ST239-III (11.1% each). SCCmec V, VI, IV and III types were detected at frequencies of 50%, 16.6%, 11.1% and 11.1%, respectively. None of the tested virulence genes were detected in all isolates, but they ranged in distribution from 1/18 to 17/18. The Panton&ndash Valentine leukocidin (PVL)-encoding genes were detected in only four isolates and were enriched in isolates causing non-severe cases. Phylogenetic analysis revealed relatedness between three ST1535-Vs, two ST5-VIs, two ST239-IIIs and two ST1-Vs however, only the two genetically related ST1-V isolates were epidemiologically linked. While disease outcome and source of infection had no correlation with a particular genotypic pattern, the sequence type was the most correlated factor with phylogeny and genotypic patterns, and a few genes were associated with non-severe cases. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4425 |
| DOI: | 10.3390/genes11101219 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f036d7c92077a7474d702650ce758723 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f036d7c92077a7474d702650ce758723 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734425 |
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| DOI: | 10.3390/genes11101219 |