أعرض في EDS

Differences in T cell cytotoxicity and cell death mechanisms between progressive multifocal leukoencephalopathy, herpes simplex virus encephalitis and cytomegalovirus encephalitis

التفاصيل البيبلوغرافية
العنوان: Differences in T cell cytotoxicity and cell death mechanisms between progressive multifocal leukoencephalopathy, herpes simplex virus encephalitis and cytomegalovirus encephalitis
المؤلفون: Ulrike Köck, Jan Bauer, Hans Lassmann, Etsuji Saji, Anna R. Tröscher, Susanne Laukoter, Kurt A. Jellinger, Helmut Rauschka
المصدر: Acta Neuropathologica
بيانات النشر: Springer Berlin Heidelberg, 2016.
سنة النشر: 2016
مصطلحات موضوعية: 0301 basic medicine, Male, viruses, T-Lymphocytes, JC virus, Cytomegalovirus, Herpes simplex virus, medicine.disease_cause, 0302 clinical medicine, Cytotoxic T cell, Cell death pathways, Child, Parthanatos, Progressive multifocal leucoencephalopathy, Neurons, Microscopy, Confocal, Cell Death, Caspase 3, Progressive multifocal leukoencephalopathy, Leukoencephalopathy, Progressive Multifocal, Apoptosis Inducing Factor, Brain, Middle Aged, Immunohistochemistry, 3. Good health, Virus encephalitis, Oligodendroglia, Cytomegalovirus Infections, Female, Adult, Clinical Neurology, Biology, Pathology and Forensic Medicine, 03 medical and health sciences, Cellular and Molecular Neuroscience, Immune system, medicine, Humans, Caspase-mediated, Aged, Original Paper, AIDS-Related Opportunistic Infections, Viral encephalitis, medicine.disease, Virology, 030104 developmental biology, Microscopy, Fluorescence, Apoptosis, Neurology (clinical), Encephalitis, Herpes Simplex, 030217 neurology & neurosurgery, CD8
الوصف: During the appearance of human immunodeficiency virus infection in the 1980 and the 1990s, progressive multifocal leukoencephalopathy (PML), a viral encephalitis induced by the JC virus, was the leading opportunistic brain infection. As a result of the use of modern immunomodulatory compounds such as Natalizumab and Rituximab, the number of patients with PML is once again increasing. Despite the presence of PML over decades, little is known regarding the mechanisms leading to death of infected cells and the role the immune system plays in this process. Here we compared the presence of inflammatory T cells and the targeting of infected cells by cytotoxic T cells in PML, herpes simplex virus encephalitis (HSVE) and cytomegalovirus encephalitis (CMVE). In addition, we analyzed cell death mechanisms in infected cells in these encephalitides. Our results show that large numbers of inflammatory cytotoxic T cells are present in PML lesions. Whereas in HSVE and CMVE, single or multiple appositions of CD8+ or granzyme-B+ T cells to infected cells are found, in PML such appositions are significantly less apparent. Analysis of apoptotic pathways by markers such as activated caspase-3, caspase-6, poly(ADP-ribose) polymerase-1 (PARP-1) and apoptosis-inducing factor (AIF) showed upregulation of caspase-3 and loss of caspase-6 from mitochondria in CMVE and HSVE infected cells. Infected oligodendrocytes in PML did not upregulate activated caspase-3 but instead showed translocation of PARP-1 from nucleus to cytoplasm and AIF from mitochondria to nucleus. These findings suggest that in HSVE and CMVE, cells die by caspase-mediated apoptosis induced by cytotoxic T cells. In PML, on the other hand, infected cells are not eliminated by the immune system but seem to die by virus-induced PARP and AIF translocation in a type of cell death defined as parthanatos. Electronic supplementary material The online version of this article (doi:10.1007/s00401-016-1642-1) contains supplementary material, which is available to authorized users.
اللغة: English
تدمد: 1432-0533
0001-6322
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f07f096e7dfbda4768cc08487c79b421
http://europepmc.org/articles/PMC5348553
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....f07f096e7dfbda4768cc08487c79b421
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14320533
00016322