Characterization of a bafinivirus exoribonuclease activity
| العنوان: | Characterization of a bafinivirus exoribonuclease activity |
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| المؤلفون: | John Ziebuhr, Marc Sauerwald, Ramakanth Madhugiri, Izabela Durzynska, Nadja Karl |
| المصدر: | Journal of General Virology. 99:1253-1260 |
| بيانات النشر: | Microbiology Society, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Gene Expression Regulation, Viral, 0301 basic medicine, Coronaviridae, RNA-dependent RNA polymerase, Virus Replication, Gene Expression Regulation, Enzymologic, Substrate Specificity, Viral Proteins, 03 medical and health sciences, chemistry.chemical_compound, Exon, Virology, RNA polymerase, Exoribonuclease, RNA, Double-Stranded, Genetics, biology, RNA, RNA virus, biology.organism_classification, 030104 developmental biology, chemistry, Exoribonucleases, RNA, Viral, Torovirinae, Exoribonuclease activity |
| الوصف: | White bream virus (WBV), a poorly characterized plus-strand RNA virus infecting freshwater fish of the Cyprinidae family, is the prototype species of the genus Bafinivirus in the subfamily Torovirinae (family Coronaviridae, order Nidovirales). In common with other nidoviruses featuring >20 kilobase genomes, bafiniviruses have been predicted to encode an exoribonuclease (ExoN) in their replicase gene. Here, we used information on the substrate specificity of bafinivirus 3C-like proteases to express WBV ExoN in an active form in Escherichia coli. The 374-residue protein displayed robust 3′-to-5′ exoribonuclease activity in the presence of Mg2+ ions and, unlike its coronavirus homologues, did not require a protein cofactor for activity. Characterization of mutant forms of ExoN provided support for predictions on putative active-site and conserved zinc-binding residues. WBV ExoN was revealed to be most active on double-stranded RNA substrates containing one or two non-paired 3′-terminal nucleotides, supporting its presumed role in increasing the fidelity of the bafinivirus RNA-dependent RNA polymerase. |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7dd8af4f7b5d84ee36fa2b30bd800cd https://doi.org/10.1099/jgv.0.001120 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f7dd8af4f7b5d84ee36fa2b30bd800cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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