Alternative reagents to antibodies in imaging applications
| العنوان: | Alternative reagents to antibodies in imaging applications |
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| المؤلفون: | Michelle Peckham, Ruth E. Hughes, Christian Tiede, Robert Bedford, Alistair Curd, Michael J. McPherson, Darren C. Tomlinson |
| المصدر: | Biophysical Reviews |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Research groups, Affimer, Biophysics, Nanotechnology, Review, Computational biology, Biology, Superresolution, Imaging, Monobody, 03 medical and health sciences, Adhiron, DARPin, 030104 developmental biology, 0302 clinical medicine, Structural Biology, Super-resolution, 030220 oncology & carcinogenesis, Molecular Biology |
| الوصف: | Antibodies have been indispensable tools in molecular biology, biochemistry and medical research. However, a number of issues surrounding validation, specificity and batch variation of commercially available antibodies have prompted research groups to develop novel non-antibody binding reagents. The ability to select highly specific monoclonal non-antibody binding proteins without the need for animals, the ease of production and the ability to site-directly label has enabled a wide variety of applications to be tested, including imaging. In this review, we discuss the success of a number of non-antibody reagents in imaging applications, including the recently reported Affimer. |
| تدمد: | 1867-2469 1867-2450 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7e159205c73b5c34c1270cb2170d552 https://doi.org/10.1007/s12551-017-0278-2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f7e159205c73b5c34c1270cb2170d552 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 18672469 18672450 |
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