Gene expression and genotoxicity in Manila clam (Ruditapes philippinarum) modulated by sediment contamination and lagoon dynamics in the Po river delta
| العنوان: | Gene expression and genotoxicity in Manila clam (Ruditapes philippinarum) modulated by sediment contamination and lagoon dynamics in the Po river delta |
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| المؤلفون: | Luigi Viganò, Alberto Izzotti, Francesco Maicu, Fabrizio Stefani, Christian Ferrarin, Nadia Casatta |
| المصدر: | Marine environmental research 142 (2018): 257–274. doi:10.1016/j.marenvres.2018.10.010 info:cnr-pdr/source/autori:Stefani F.; Casatta N.; Ferrarin C.; Izzotti A.; Maicu F.; Viganò L./titolo:Gene expression and genotoxicity in Manila clam (Ruditapes philippinarum) modulated by sediment contamination and lagoon dynamics in the Po river delta/doi:10.1016%2Fj.marenvres.2018.10.010/rivista:Marine environmental research/anno:2018/pagina_da:257/pagina_a:274/intervallo_pagine:257–274/volume:142 |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Delta, Geologic Sediments, Salinity, Osmotic shock, Ruditapes, 010501 environmental sciences, Aquatic Science, Oceanography, medicine.disease_cause, 01 natural sciences, Lagoon sediments, 03 medical and health sciences, Rivers, Ruditapes philippinarum, Water Movements, medicine, Animals, Lagoon modelling, 0105 earth and related environmental sciences, Po river, geography, River delta, geography.geographical_feature_category, biology, Contaminant mixtures, Chemistry, Temperature, DNA adducts, DNA, General Medicine, Models, Theoretical, Contamination, Gene expression, Oxidative stress, biology.organism_classification, Pollution, Bivalvia, 030104 developmental biology, Gene Expression Regulation, Environmental chemistry, Gene expressions, Water Pollutants, Chemical, Genotoxicity, Environmental Monitoring |
| الوصف: | The lagoons of the Po River delta are potentially exposed to complex mixtures of contaminants, nevertheless, there is a substantial lack of information about the biological effects of these contaminants in the Po delta lagoons. These environments are highly dynamic and the interactions between chemical and environmental stressors could prevent the proper identification of biological effects and their causes. In this study, we aimed to disentangle such interactions focusing on Manila clams, previously exposed to six lagoons of the Po delta, adopting three complementary tools: a) the detailed description via modelling techniques of lagoon dynamics for salinity and water temperature; b) the response sensitivity of a number of target genes (ahr, cyp4, ρ-gst, σ-gst, hsp22, hsp70, hsp90, ikb, dbh, ach, cat, Mn-sod, Cu/Zn-sod, cyp-a, flp, grx, TrxP) investigated in clam digestive glands by Real Time PCR; and c) the relevance of DNA adducts determined in clams as markers of exposure to genotoxic chemicals. The lagoons showed specific dynamics, and two of them (Marinetta and Canarin) could induce osmotic stress. A group of genes (ahr, cyp4, Mn-sod, σ-gst, hsp-22, cyp-a, TrxP) seemed to be associated with overall lagoon characteristics as may be described by salinity and its variations. Lagoon modelling and a second group of genes (hsp70, hsp90, cat, ikb, ach, grx, Cu/Zn-sod) also suggested that moderate increases of river discharge may imply worse exposure conditions. Oxidative stress seemed to be associated with such events but it was slightly evident also under normal exposure conditions. DNA adduct formation was mainly associated with overwhelmed antioxidant defences (e.g. low Cu/Zn-sod) or seemingly with their lack of response in due time. In Po delta lagoons, Manila clam can be affected by chemical and environmental factors which can contribute to induce oxidative stress, DNA adduct formation and, ultimately, to affect clam condition and health. |
| تدمد: | 0141-1136 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8331b84e409cb42a4d5bc75e9f6285d https://doi.org/10.1016/j.marenvres.2018.10.010 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....f8331b84e409cb42a4d5bc75e9f6285d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01411136 |
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