التفاصيل البيبلوغرافية
| العنوان: |
Analysis of Sublethal Toxicity in Developing Zebrafish Embryos Exposed to a Range of Petroleum Substances |
| المؤلفون: |
Bryan M. Hedgpeth, Rebecca A. Alyea, Martin J. Connelly, Aaron D. Redman, Heping Zhou, Mark A. Lampi, Josh D. Butler, Daniel J. Letinski |
| المصدر: |
Environmental Toxicology and Chemistry |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
animal structures, Embryo, Nonmammalian, Bioavailability, Health, Toxicology and Mutagenesis, Aquatic toxicology, Biomimetic extraction–solid‐phase microextraction, Embryonic Development, 010501 environmental sciences, Ecotoxicology, 01 natural sciences, Andrology, 03 medical and health sciences, Edema, Swim bladder, medicine, Toxicity Tests, Acute, Environmental Chemistry, Animals, Yolk sac, Zebrafish, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, biology, Chemistry, Fish embryo acute toxicity test, Environmental Exposure, biology.organism_classification, Environmental Toxicology, Acute toxicity, Polycyclic aromatic hydrocarbons, medicine.anatomical_structure, Petroleum, Environmental toxicology, Toxicity, embryonic structures, medicine.symptom, Water Pollutants, Chemical |
| الوصف: |
The Organisation for Economic Co‐operation and Development (OECD) test guideline 236 (fish embryo acute toxicity test; 2013) relies on 4 endpoints to describe exposure‐related effects (coagulation, lack of somite formation, tail‐bud detachment from the yolk sac, and the presence of a heartbeat). Danio rerio (zebrafish) embryos were used to investigate these endpoints along with a number of additional sublethal effects (cardiac dysfunction, pericardial edema, yolk sac edema, tail curvature, hatch success, pericardial edema area, craniofacial malformation, swim bladder development, fin development, and heart rate) following 5‐d exposures to 7 petroleum substances. The substances investigated included 2 crude oils, 3 gas oils, a diluted bitumen, and a petrochemical containing a mixture of branched alcohols. Biomimetic extraction–solid‐phase microextraction (BE–SPME) was used to quantify freely dissolved concentrations of test substances as the exposure metric. The results indicated that the most prevalent effects observed were pericardial and yolk sac edema, tail curvature, and lack of embryo viability. A BE–SPME threshold was determined to characterize sublethal morphological alterations that preceded embryo mortality. Our results aid in the understanding of aquatic hazards of petroleum substances to developing zebrafish beyond traditional OECD test guideline 236 endpoints and show the applicability of BE–SPME as a simple analytical tool that can be used to predict sublethal embryo toxicity. Environ Toxicol Chem 2019;38:1302–1312. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC. |
| تدمد: |
1552-8618 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f83a33d4aada073d9284c64908144db8
https://pubmed.ncbi.nlm.nih.gov/30919522 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....f83a33d4aada073d9284c64908144db8 |
| قاعدة البيانات: |
OpenAIRE |
