Clinicobiological, immunophenotypic, and molecular characteristics of monoclonal CD56‐/+dim chronic natural killer cell large granular lymphocytosis
| العنوان: | Clinicobiological, immunophenotypic, and molecular characteristics of monoclonal CD56‐/+dim chronic natural killer cell large granular lymphocytosis |
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| المؤلفون: | Alexandra Estevinho, Ana Helena Santos, Alberto Orfao, Maria dos Anjos Teixeira, Marcos González, Luciana Pinho, Paloma Bárcena, Sónia Fonseca, Ana Balanzategui, Maria del Carmen Alguero, José Manuel Cabeda, Benvindo Justiça, Maria Luís Queirós, Jesús F. San Miguel, Margarida Lima, Julia Almeida, Maria Luís Amorim, Andrés Celestino García Montero |
| المصدر: | CIÊNCIAVITAE Scopus-Elsevier Europe PubMed Central Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| بيانات النشر: | American Society for Investigative Pathology (ASIP), 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Adult, Male, Lymphocytosis, genetic structures, Lymphoproliferative disorders, chemical and pharmacologic phenomena, Biology, Gene Rearrangement, T-Lymphocyte, Polymerase Chain Reaction, Pathology and Forensic Medicine, Natural killer cell, Immunophenotyping, Neoplasms, medicine, Biomarkers, Tumor, Humans, Aged, Aged, 80 and over, hemic and immune systems, Gene rearrangement, Middle Aged, medicine.disease, Flow Cytometry, Molecular biology, Hematologic Diseases, CD56 Antigen, Granzyme B, Killer Cells, Natural, Blotting, Southern, medicine.anatomical_structure, Virus Diseases, Immunology, Chronic Disease, Cytokines, Tumor necrosis factor alpha, Female, sense organs, medicine.symptom, CD8, Regular Articles |
| الوصف: | Am J Pathol. 2004 Oct;165(4):1117-27. Clinicobiological, immunophenotypic, and molecular characteristics of monoclonal CD56-/+dim chronic natural killer cell large granular lymphocytosis. Lima M, Almeida J, Montero AG, Teixeira Mdos A, Queirós ML, Santos AH, Balanzategui A, Estevinho A, Algueró Mdel C, Barcena P, Fonseca S, Amorim ML, Cabeda JM, Pinho L, Gonzalez M, San Miguel J, Justiça B, Orfão A. Serviço de Hematologia, Unidade de Citometria, Hospital Geral de Santo António, Rua D Manuel II, s/n, 4099-001 Porto, Portugal. mmc.lima@clix.pt. Abstract Indolent natural killer (NK) cell lymphoproliferative disorders include a heterogeneous group of patients in whom persistent expansions of mature, typically CD56(+), NK cells in the absence of any clonal marker are present in the peripheral blood. In the present study we report on the clinical, hematological, immunophenotypic, serological, and molecular features of a series of 26 patients with chronic large granular NK cell lymphocytosis, whose NK cells were either CD56(-) or expressed very low levels of CD56 (CD56(-/+dim) NK cells), in the context of an aberrant activation-related mature phenotype and proved to be monoclonal using the human androgen receptor gene polymerase chain reaction-based assay. As normal CD56(+) NK cells, CD56(-/+dim) NK cells were granzyme B(+), CD3(-), TCRalphabeta/gammadelta(-), CD5(-), CD28(-), CD11a(+bright), CD45RA(+bright), CD122(+), and CD25(-) and they showed variable and heterogeneous expression of both CD8 and CD57. Nevertheless, they displayed several unusual immunophenotypic features. Accordingly, besides being CD56(-/+dim), they were CD11b(-/+dim) (heterogeneous), CD7(-/+dim) (heterogeneous), CD2(+) (homogeneous), CD11c(+bright) (homogeneous), and CD38(-/+dim) (heterogeneous). Moreover, CD56(-/+dim) NK cells heterogeneously expressed HLA-DR. In that concerning the expression of killer receptors, CD56(-/+dim) NK cells showed bright and homogeneous CD94 expression, and dim and heterogeneous reactivity for CD161, whereas CD158a and NKB1 expression was variable. From the functional point of view, CD56(-/+dim) showed a typical Th1 pattern of cytokine production (interferon-gamma(+), tumor necrosis factor-alpha(+)). From the clinical point of view, these patients usually had an indolent clinical course, progression into a massive lymphocytosis with lung infiltration leading to death being observed in only one case. Despite this, they frequently had associated cytopenias as well as neoplastic diseases and/or viral infections. In summary, we describe a unique and homogeneous group of monoclonal chronic large granular NK cell lymphocytosis with an aberrant activation-related CD56(-/+dim)/CD11b(-/+dim) phenotype and an indolent clinical course, whose main clinical features are related to concomitant diseases. PMID: 15466379 [PubMed - indexed for MEDLINE]PMCID: PMC1618630 |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8da836402a338077ddde7d5bc247b63 https://hdl.handle.net/10400.16/567 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f8da836402a338077ddde7d5bc247b63 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |