Development of a high‐resolution typing method for SLA‐3, swine MHC class I antigen 3
| العنوان: | Development of a high‐resolution typing method for SLA‐3, swine MHC class I antigen 3 |
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| المؤلفون: | S. Youk, M.T. Le, M. Kang, B. Ahn, M. Choi, K. Kim, T.H. Kim, J.H. Kim, C.S. Ho, C. Park |
| المصدر: | Animal Genetics. 53:166-170 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genotyping Techniques, Histocompatibility Antigens Class I, Sus scrofa, Genetics, Animals, Animal Science and Zoology, Sequence Analysis, DNA, General Medicine |
| الوصف: | We developed a high-resolution and comprehensive typing method for swine leukocyte antigen 3 (SLA-3), an MHC class I gene, employing locus-specific genomic PCR followed by subsequent direct sequencing. A total of 292 individuals from nine pure, one cross-breed and six cell lines were successfully typed. A total of 21 SLA-3 alleles were identified, of which four were found to be novel alleles. However, the allelic diversity of SLA-3 was lower than that of previously reported class I genes, SLA-1 and -2. More SLA-3 alleles were observed in the Landrace and Yorkshire breeds than the other breeds. SLA-3*04:01 was identified in seven out of nine breeds and was the most widely distributed allele across all breeds. Therefore, the typing method reported in this study completes our efforts to develop high-resolution typing methods for major SLA molecules, facilitating the combined analysis of major SLA genes from field samples, which is important to understand the relationship between the adaptive immune responses against pathogens and the immunogenetic makeup of an individual. |
| تدمد: | 1365-2052 0268-9146 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc0a672f659b94304f472d028e924306 https://doi.org/10.1111/age.13161 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....fc0a672f659b94304f472d028e924306 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652052 02689146 |
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