التفاصيل البيبلوغرافية
| العنوان: |
New potential peptide therapeutics perturbing CK1δ/α-tubulin interaction |
| المؤلفون: |
Krüger, M. Kalbacher, H. Kastritis, P.L. Bischof, J. Barth, H. Henne-Bruns, D. Vorgias, C. Sarno, S. Pinna, L.A. Knippschild, U. |
| سنة النشر: |
2016 |
| الوصف: |
Members of the CK1 family are highly conserved serine/threonine specific kinases being expressed in all eukaryotes. They are involved in many cellular processes and therefore tightly regulated. A central mechanism to modulate CK1 activity is via interaction with cellular proteins. CK1δ interacts with α-/β-tubulin and is involved in the regulation of microtubule dynamics. Therefore, it is important to identify the structural elements responsible for the interaction between these proteins. Using a peptide library covering the human CK1δ amino acid sequence in SPR and ELISA analyses, we identified peptide 39 (P39), encompassing aa361-aa375 of CK1δ, as a prominent binding partner of α-tubulin. P39 decreases α-tubulin phosphorylation by CK1δ and reduces the thermodynamic stability of α-tubulin in fluorescence thermal shift assays. Furthermore, P39 induces an inhibition of mitotic progression and a disruption of cells entering mitosis in CV-1 cells. Taken together our data provide valuable information regarding the interaction of CK1δ and α-tubulin and a novel approach for the development of pharmacological tools to inhibit proliferation of cancer cells. © 2016 Elsevier Ireland Ltd. |
| اللغة: |
English |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=od______2127::ef3394706598d6f3e740315ed841b537
https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3087033 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.od......2127..ef3394706598d6f3e740315ed841b537 |
| قاعدة البيانات: |
OpenAIRE |
