Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell
| العنوان: | Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell |
|---|---|
| المؤلفون: | Sherry, Boozer, Nicholas, Lehman, Uma, Lakshmipathy, Brad, Love, Amy, Raber, Anirban, Maitra, Robert, Deans, Mahendra S, Rao, Anthony E, Ting |
| المصدر: | Journal of stem cells. 4(1) |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult, Adult Stem Cells, Bone Marrow, Multipotent Stem Cells, Cell Culture Techniques, Humans, Bone Marrow Cells, Cell Differentiation, Genomic Instability |
| الوصف: | The therapeutic benefits of adult adherent stem cells are currently being investigated in clinical trials for a variety of diseases. Data from initial clinical studies are promising and as a consequence of moving to later stage clinical studies, have resulted in larger scale clinical-grade cell production strategies. Therefore it becomes imperative to examine the epigenetic flux and genomic stability of stem cells in long-term culture to determine that minimal risk is associated with these therapies. Multipotent adult progenitor cells (MAPC) are an adherent adult stem cell population that can be derived from bone marrow and was the first of a class of adult stem cells that have broad developmental potential both in vitro and in vivo. Here, we report a panel of tests to characterize MultiStem, a multipotent adult stem cell type based on MAPC, and establish its genomic stability during culture expansion. A variety of techniques were employed that consisted of miRNA expression to characterize and define the cell population; chromosomal SNP analysis and G-banding to determine karyotypic stability; and methylation pattern and telomerase expression to examine potential changes in epigenetic and chromosomal stability with prolonged in vitro culture of cells. This panel of test was applied to cultures at early isolation stages and compared to cultures harvested at population doublings greater than those reached in current MultiStem clinical trials. These tests also provide a baseline for quality control of cells prepared from various biological donor sources for subsequent large scale propagation and preparation of cell banks for downstream applications. |
| تدمد: | 1556-8539 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::376f2e8a09f784e4e41cdc3ca7ea8fde https://pubmed.ncbi.nlm.nih.gov/20498688 |
| رقم الأكسشن: | edsair.pmid..........376f2e8a09f784e4e41cdc3ca7ea8fde |
| قاعدة البيانات: | OpenAIRE |
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