An Introduction to Advanced Targeted Acquisition Methods
| العنوان: | An Introduction to Advanced Targeted Acquisition Methods |
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| المؤلفون: | van Bentum, Mirjam, Selbach, Matthias |
| المصدر: | Molecular & Cellular Proteomics : MCP |
| بيانات النشر: | American Society for Biochemistry and Molecular Biology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | RT, retention time, SIL, stable isotope labeled, Proteomics, targeted proteomics, SRM, TOMAHAQ, triggered-by-offset, multiplexed, accurate-mass, high-resolution, and absolute quantification, SILAC, stable isotope labeling by amino acids in cell culture, PRM, DIA, data-independent acquisition, Review, maxIT, maximum injection time or fill time, Mass Spectrometry, PRM, parallel reaction monitoring, Animals, Humans, SRM, selected reaction monitoring, QqTOF, quadrupole-TOF, MaxQuant.Live, LOQ, limit of quantification, IS-PRM, LOD, limit of detection, iRT, TOMAHAQ, iRT, indexed retention time, TMT, tandem mass tag, Picky, DDA, data-dependent acquisition, QqQ, triple quadrupole mass spectrometer, MRM, QqOrbi, quadrupole-Orbitrap, Peptides, SureQuant, MRM, multiple reaction monitoring |
| الوصف: | Targeted proteomics via selected reaction monitoring (SRM) or parallel reaction monitoring (PRM) enables fast and sensitive detection of a preselected set of target peptides. However, the number of peptides that can be monitored in conventional targeting methods is usually rather small. Recently, a series of methods has been described that employ intelligent acquisition strategies to increase the efficiency of mass spectrometers to detect target peptides. These methods are based on one of two strategies. First, retention time adjustment-based methods enable intelligent scheduling of target peptide retention times. These include Picky, iRT, as well as spike-in free real-time adjustment methods such as MaxQuant.Live. Second, in spike-in triggered acquisition methods such as SureQuant, Pseudo-PRM, TOMAHAQ, and Scout-MRM, targeted scans are initiated by abundant labeled synthetic peptides added to samples before the run. Both strategies enable the mass spectrometer to better focus data acquisition time on target peptides. This either enables more sensitive detection or a higher number of targets per run. Here, we provide an overview of available advanced targeting methods and highlight their intrinsic strengths and weaknesses and compatibility with specific experimental setups. Our goal is to provide a basic introduction to advanced targeting methods for people starting to work in this field. Graphical abstract Highlights • Advanced acquisition methods improve focus of mass spectrometers on target peptides. • This review discusses existing methods based on two strategies. • Retention time adjustment-based methods enable intelligent scheduling of peptide RTs. • In spike-in triggered acquisition methods targeted scans are initiated by spike-ins. In Brief The analytical power of targeted proteomics depends on how efficiently the mass spectrometer detects target peptides. A number of “smart” acquisition approaches have been developed that enable more targets per run and improve analytical performance such as sensitivity, specificity, and quantitative accuracy. This review provides an introduction to these methods and highlights their inherent strengths and weaknesses. |
| اللغة: | English |
| تدمد: | 1535-9484 1535-9476 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::715ed789b7332d8a2280f1721a4d70da http://europepmc.org/articles/PMC8600983 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid..........715ed789b7332d8a2280f1721a4d70da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15359484 15359476 |
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