The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies
العنوان: | The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies |
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المؤلفون: | Somchai, Sangkitporn, Parichat, Pung-amritt, Siripakorn K, Sangkitporn, Areerat, Sangnoi, Voravarn S, Tanphaichitr |
المصدر: | The Southeast Asian journal of tropical medicine and public health. 33(4) |
سنة النشر: | 2003 |
مصطلحات موضوعية: | Genetic Carrier Screening, Hemoglobin E, Homozygote, Immunoblotting, Thailand, Polymerase Chain Reaction, Sensitivity and Specificity, Hemoglobinopathies, Automation, Phenotype, Bias, Case-Control Studies, Linear Models, Humans, Thalassemia, Hemoglobin A2, Fetal Hemoglobin, Chromatography, Liquid |
الوصف: | Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of the thalassemias and hemoglobinopathies. Presented in this study is the validation of the the Hb A2 assay of the HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV's of within-run, between-run and interlaboratory studies were 1.8-3.1%, 3.4-6.0% and 6.8-8.8% respectively. The results of the %Hb A2 quantitated by HbGold analyzer correlated well with those given by the Bio-Rad Variant Hb testing system (r=0.98). The application of the HbGold analyzer for the diagnosis of the thalassemia phenotypes frequently observed in Thailand is considered. In conclusion, the Hb A2 assay of the HbGold analyzer could be used for the quantitation of Hb A2 and Hb F and the presumptive identification of abnormal hemoglobins. |
تدمد: | 0125-1562 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::8dbefdae80f9f55447d1f475e81f70e7 https://pubmed.ncbi.nlm.nih.gov/12757240 |
رقم الأكسشن: | edsair.pmid..........8dbefdae80f9f55447d1f475e81f70e7 |
قاعدة البيانات: | OpenAIRE |
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