[Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)]

التفاصيل البيبلوغرافية
العنوان: [Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)]
المؤلفون: Jian-hui, Zhou, Xiang, Hou, Chao, Chen, Shuang, Wang, Xin, Chang, Gui-yan, Liu, Zhao-nan, Wang, Song-tao, Xu, Yi-xin, Ji, Wen-bo, Xu
المصدر: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology. 22(6)
سنة النشر: 2009
مصطلحات موضوعية: Measles virus, Reverse Transcriptase Polymerase Chain Reaction, Humans, Genome, Viral, Measles
الوصف: A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.RT-LAMP is more sensitive than nest-RT-PCR.
تدمد: 1003-9279
URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::9c31d229cd66b73c1f19a398f17a60ae
https://pubmed.ncbi.nlm.nih.gov/19544627
رقم الأكسشن: edsair.pmid..........9c31d229cd66b73c1f19a398f17a60ae
قاعدة البيانات: OpenAIRE