Printing low density protein arrays in microplates
| العنوان: | Printing low density protein arrays in microplates |
|---|---|
| المؤلفون: | Robert S, Matson, Raymond C, Milton, Michael C, Cress, Tom S, Chan, Jang B, Rampal |
| المصدر: | Methods in molecular biology (Clifton, N.J.). 381 |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Mice, Protein Array Analysis, Animals, Cytokines, Humans, Enzyme-Linked Immunosorbent Assay, Antibodies, Software |
| الوصف: | Here, we provide methods for the creation of protein microarrays in microplates. The microplate consists of 96 wells with each well capable of holding a protein microarray at a spot density of up to 400 (20 x 20) individual elements. Arrays of capture monoclonal antibodies, corresponding to specific interleukins, were printed onto the bottom of the wells which had been surface activated for covalent attachment. A Biomek 2000 laboratory automation workstation (Beckman Coulter, Inc., Fullerton, CA) equipped with a high-density replicating tool was used for printing low density 3 x 3 to 5 x 5 arrays. For higher density arrays, a microarrayer system (Cartesian PS7200, Genomic Solutions, Inc., Ann Arbor, MI) was employed. Multiple antigens were simultaneously analyzed without detectable cross-reactivity associated with capture antibody or secondary antibody interactions. Detection of interleukin antigens, spiked into cell culture media containing 10% fetal calf serum, was specific and sensitive. |
| تدمد: | 1064-3745 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::9f45ff17b6552a92c026edff8803a974 https://pubmed.ncbi.nlm.nih.gov/17984528 |
| رقم الأكسشن: | edsair.pmid..........9f45ff17b6552a92c026edff8803a974 |
| قاعدة البيانات: | OpenAIRE |
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