Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex
| العنوان: | Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex |
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| المؤلفون: | Zhang, ZQ, Chun, ACS, Chin, KT, Jeang, KT, Jin, DY, Ching, YP |
| المصدر: | Retrovirology Retrovirology, Vol 1, Iss 1, p 18 (2004) |
| بيانات النشر: | BioMed Central, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, Human T-lymphotropic virus 1, Transcription, Genetic, Histocompatibility antigens - genetics, viruses, Research, Nuclear Proteins, RNA-Binding Proteins, Gene Products, tax, Antigens, Neoplasm, Histocompatibility Antigens, Gene products, tax - metabolism, Humans, Antigens, neoplasm - genetics, lcsh:RC581-607, Cyclic AMP Response Element-Binding Protein, Peptide Chain Initiation, Translational, Promoter Regions, Genetic, Human t-lymphotropic virus 1 - genetics, Repetitive Sequences, Nucleic Acid, Transcription Factors |
| الوصف: | Background: Human T-cell leukemia virus type I (HTLV-I) Tax protein is a transcriptional regulator of viral and cellular genes. In this study we have examined in detail the determinants for Tax-mediated transcriptional activation. Results: Whereas previously the LTR enhancer elements were thought to be the sole Tax-targets, herein, we find that the core HTLV-I TATAA motif also provides specific responsiveness not seen with either the SV40 or the E1b TATAA boxes. When enhancer elements which can mediate Tax-responsiveness were compared, the authentic HTLV-I 21-bp repeats were found to be the most effective. Related bZIP factors such as CREB, ATF4, c-Jun and LZIP are often thought to recognize the 21-bp repeats equivalently. However, amongst bZIP factors, we found that CREB, by far, is preferred by Tax for activation. When LTR transcription was reconstituted by substituting either κB or serum response elements in place of the 21-bp repeats, Tax activated these surrogate motifs using surfaces which are different from that utilized for CREB interaction. Finally, we employed artificial recruitment of TATA-binding protein to the HTLV-I promoter in "bypass" experiments to show for the first time that Tax has transcriptional activity subsequent to the assembly of an initiation complex at the promoter. Conclusions: Optimal activation of the HTLV-I LTR by Tax specifically requires the core HTLV-I TATAA promoter, CREB and the 21-bp repeats. In addition, we also provide the first evidence for transcriptional activity of Tax after the recruitment of TATA-binding protein to the promoter. © 2004 Ching et al; licensee BioMed Central Ltd. published_or_final_version |
| وصف الملف: | 1175820 bytes; 25088 bytes; application/pdf; application/msword |
| اللغة: | English |
| تدمد: | 1742-4690 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::1782be6e56ad8967fa4ba4d8c13cf491 http://europepmc.org/articles/PMC509288 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....1782be6e56ad8967fa4ba4d8c13cf491 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17424690 |
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