تقرير
Nonequilibrium thermodynamics of DNA nanopore unzipping
| العنوان: | Nonequilibrium thermodynamics of DNA nanopore unzipping |
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| المؤلفون: | Suma, Antonio, Carnevale, Vincenzo, Micheletti, Cristian |
| سنة النشر: | 2022 |
| المجموعة: | Condensed Matter Physics (Other) Quantitative Biology |
| مصطلحات موضوعية: | Condensed Matter - Soft Condensed Matter, Physics - Biological Physics, Quantitative Biology - Biomolecules |
| الوصف: | Using theory and simulations, we carried out a first systematic characterization of DNA unzipping via nanopore translocation. Starting from partially unzipped states, we found three dynamical regimes depending on the applied force, f: (i) heterogeneous DNA retraction and rezipping (f < 17pN), (ii) normal (17pN < f < 60pN) and (iii) anomalous (f > 60pN) drift-diffusive behavior. We show that the normal drift-diffusion regime can be effectively modelled as a one-dimensional stochastic process in a tilted periodic potential. We use the theory of stochastic processes to recover the potential from nonequilibrium unzipping trajectories and show that it corresponds to the free-energy landscape for single base-pairs unzipping. Applying this general approach to other single-molecule systems with periodic potentials ought to yield detailed free-energy landscapes from out-of-equilibrium trajectories. Comment: 6 pages, 4 figures |
| نوع الوثيقة: | Working Paper |
| DOI: | 10.1103/PhysRevLett.130.048101 |
| URL الوصول: | http://arxiv.org/abs/2212.05882 |
| رقم الأكسشن: | edsarx.2212.05882 |
| قاعدة البيانات: | arXiv |
| DOI: | 10.1103/PhysRevLett.130.048101 |
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