دورية أكاديمية
Lactoferrin impact on gut microbiota in preterm infants with late-onset sepsis or necrotising enterocolitis: the MAGPIE mechanisms of action study
| العنوان: | Lactoferrin impact on gut microbiota in preterm infants with late-onset sepsis or necrotising enterocolitis: the MAGPIE mechanisms of action study |
|---|---|
| المؤلفون: | Nicholas Embleton, Janet Berrington, Stephen Cummings, Jon Dorling, Andrew Ewer, Alessandra Frau, Edmund Juszczak, John Kirby, Christopher Lamb, Clare Lanyon, Lauren Lett, William McGuire, Christopher Probert, Stephen Rushton, Mark Shirley, Christopher Stewart, Gregory R Young |
| المصدر: | Efficacy and Mechanism Evaluation, Vol 8, Iss 14 (2021) |
| بيانات النشر: | NIHR Journals Library, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | humans, infant, infant, newborn, enterocolitis, necrotizing, ltf protein, human, lactoferrin, gastrointestinal microbiome, dysbiosis, enteral nutrition, risk factors, milk, metabolome, high-throughput nucleotide sequencing, infant, premature, sepsis, feces, fatty acids, volatile, immune system, mass spectrometry, clinical protocols, Medicine |
| الوصف: | Background: Preterm infants have high rates of morbidity, especially from late-onset sepsis and necrotising enterocolitis. Lactoferrin is an anti-infective milk protein that may act through effects on gut bacteria, metabolites and epithelial cell function. The impact of supplemental lactoferrin in reducing late-onset sepsis was explored in the Enteral LactoFerrin In Neonates (ELFIN) trial. Objectives: The Mechanisms Affecting the Gut of Preterm Infants in Enteral feeding (MAGPIE) study was nested within the ELFIN trial and aimed to determine the impact of lactoferrin on gut microbiota and bacterial function, and changes preceding disease onset. We aimed to explore impacts on the stool bacteria and faecal/urinary metabolome using gas and liquid chromatography–mass spectrometry, and explore immunohistological pathways in resected tissue. Methods: Preterm infants from 12 NHS hospitals were enrolled in the study, and daily stool and urine samples were collected. Local sample collection data were combined with ELFIN trial data from the National Perinatal Epidemiology Unit, Oxford. The longitudinal impact of lactoferrin in healthy infants was determined, and samples that were collected before disease onset were matched with samples from healthy control infants. Established, quality-controlled 16S ribonucleic acid, gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry analyses were conducted. Validated databases and standardised workflows were used to identify bacteria and metabolites. Tissue samples from infants undergoing surgery and matched controls were analysed. Results: We recruited 479 preterm infants (mean gestation of 28.4 ± 2.3 weeks) and collected > 33,000 usable samples from 467 infants. 16S ribonucleic acid bacterial analysis was conducted on samples from 201 infants, of whom 20 had necrotising enterocolitis and 51 had late-onset sepsis, along with samples from healthy matched controls to explore longitudinal changes. The greatest change in relative bacterial abundance over time was observed in Staphylococcus, which decreased from 42% at aged 7–9 days to only 2% at aged 30–60 days (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-4365 2050-4373 |
| Relation: | https://doaj.org/toc/2050-4365; https://doaj.org/toc/2050-4373 |
| DOI: | 10.3310/eme08140 |
| URL الوصول: | https://doaj.org/article/006be589aebd4743af53271f679dc81b |
| رقم الأكسشن: | edsdoj.006be589aebd4743af53271f679dc81b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20504365 20504373 |
|---|---|
| DOI: | 10.3310/eme08140 |