دورية أكاديمية
Inducible modulation of miR-204 levels in a zebrafish melanoma model
| العنوان: | Inducible modulation of miR-204 levels in a zebrafish melanoma model |
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| المؤلفون: | Samanta Sarti, Raffaella De Paolo, Chiara Ippolito, Angela Pucci, Letizia Pitto, Laura Poliseno |
| المصدر: | Biology Open, Vol 9, Iss 11 (2020) |
| بيانات النشر: | The Company of Biologists, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | zebrafish, melanoma model, minicoopr-i, mir-204, pigmentation, Science, Biology (General), QH301-705.5 |
| الوصف: | Here, we present miniCoopR-I, an inducible upgrade of the constitutive miniCoopR vector. We developed miniCoopR-I-sponge-204 and miniCoopR-I-pre-miR-204 vectors and we successfully tested them for their ability to achieve time- (embryo/juvenile/adult) and space- (melanocytic lineage) restricted inhibition/overexpression of miR-204, a positive modulator of pigmentation previously discovered by us. Furthermore, melanoma-free survival curves performed on induced fish at the adult stage indicate that miR-204 overexpression accelerates the development of BRAFV600E-driven melanoma. miniCoopR-I allows study of the impact that coding and non-coding modulators of pigmentation exert on melanomagenesis in adult zebrafish, uncoupling it from the impact that they exert on melanogenesis during embryonic development. This article has an associated First Person interview with the first author of the paper. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| Relation: | http://bio.biologists.org/content/9/11/bio053785; https://doaj.org/toc/2046-6390 |
| DOI: | 10.1242/bio.053785 |
| URL الوصول: | https://doaj.org/article/d02bdc6d4afd43768f3c049f1dfbcdf9 |
| رقم الأكسشن: | edsdoj.02bdc6d4afd43768f3c049f1dfbcdf9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
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| DOI: | 10.1242/bio.053785 |