دورية أكاديمية
Enhancement of xylanase expression by Komagataella phaffii through pexophagy inhibition
| العنوان: | Enhancement of xylanase expression by Komagataella phaffii through pexophagy inhibition |
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| المؤلفون: | Taiyu Liu, Yixin Zhao, Jianguo Zhang, Jining Zhang |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 855-862 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | gene knockout, komagataella phaffii, mixed carbon source feeding, xylanase, Biotechnology, TP248.13-248.65 |
| الوصف: | Xylanase is widely used in agriculture and food industry. To increase heterologous xylanase expression by Komagataella phaffii under the condition of mixed carbon source feeding, the atg30 gene was knocked out from this yeast to inhibit pexophagy for increasing sorbitol consumption. Komagataella phaffii atg30Δ was constructed through homologous recombination that resulted in the inhibition of pexophagy. When compared with the control, the yield of secretory xylanase increased by 4.1 ± 0.2%, 5.7 ± 0.5%, 11.8 ± 0.4% and 11.4 ± 0.7% under the conditions of 1.5% methanol and 0.5, 1, 1.5 and 2% sorbitol addition, respectively. The residual sorbitol analysis showed that sorbitol consumed by Komagataella phaffii atg30Δ increased by 121.3 ± 9.3%, 66.8 ± 15.2% and 31.2 ± 3.2% following 1, 1.5 and 2 sorbitol addition, respectively. Thus, non-methanol carbon source, co-feeding with methanol, provided more energy without pexophagy by atg30 gene knockout. Pexophagy inhibition increased the heterologous protein expression by Komagataella phaffii, which provided basis for increased yield of xylanase through pexophagy monitoring. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2019.1623717 |
| URL الوصول: | https://doaj.org/article/d06ff408ed8243fbbde19558983dc86b |
| رقم الأكسشن: | edsdoj.06ff408ed8243fbbde19558983dc86b |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2019.1623717 |