دورية أكاديمية
CRISPR/Cas9 as a Mutagenic Factor
| العنوان: | CRISPR/Cas9 as a Mutagenic Factor |
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| المؤلفون: | Andrey R. Shumega, Youri I. Pavlov, Angelina V. Chirinskaite, Aleksandr A. Rubel, Sergey G. Inge-Vechtomov, Elena I. Stepchenkova |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 2, p 823 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | genome editing, CRISPR/Cas9, mutations, off-target activity, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites. DNA repair systems differing in accuracy participate in establishing desired genetic changes but also introduce unwanted mutations, that may lead to hereditary, oncological, and other diseases. New approaches to alleviate the risks associated with genome editing include attenuating the off-target activity of editing complex through the use of modified forms of Cas9 nuclease and single guide RNA (sgRNA), improving delivery methods for sgRNA/Cas9 complex, and directing DNA lesions caused by the sgRNA/Cas9 to non-mutagenic repair pathways. Here, we have described CRISPR/Cas9 as a new powerful mutagenic factor, discussed its mutagenic properties, and reviewed factors influencing the mutagenic activity of CRISPR/Cas9. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/2/823; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25020823 |
| URL الوصول: | https://doaj.org/article/07be874c5a7645519555fa5d5937a372 |
| رقم الأكسشن: | edsdoj.07be874c5a7645519555fa5d5937a372 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25020823 |