دورية أكاديمية
Key genes of inflammation and Crohn’s disease severity identified via bioinformatics and clinical specimen analysis
| العنوان: | Key genes of inflammation and Crohn’s disease severity identified via bioinformatics and clinical specimen analysis |
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| المؤلفون: | Min Lu, Zhirui Zeng, Yongjian Ling, Qinsong Zhong, Zhiliang Mai, Hua Mao, Qian Wang |
| المصدر: | All Life, Vol 16, Iss 1 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Life |
| مصطلحات موضوعية: | crohn’s disease, weighted gene co-expression network analysis, differentially expressed gene, biomarkers, slc39a5, Biotechnology, TP248.13-248.65, Life, QH501-531 |
| الوصف: | Crohn’s disease (CD) is a nonspecific inflammatory disease of digestive tract with limited known biomarkers. We aimed to identify potential molecules involved in CD severity. Weighted gene co-expression network and differentially expressed gene analyses were used to explore hub genes associated with inflammation and involved in pathological processes of CD in the GSE83448 cohort. The expression and relationship of genes with clinical characteristics in normal and CD intestinal mucosa were validated in GSE208303 and our research cohorts. Five genes related to disease and inflammation were identified in the clinically significant modules that were differentially expressed in healthy controls and CD patients. Among them, solute carrier family 39 member 5 (SLC39A5) expression was reduced in CD tissues compared with that in normal tissues; low SLC39A5 expression was associated with CD-simplifying endoscopic score and stenosis. Furthermore, we broadened the bioinformatic threshold with qRT-PCR verification and western blot, and found that the expressions of cytochrome P450 family 2 subfamily S member 1 (CYP2S1), fatty acid 2-hydroxylase (FA2H), fatty acid binding protein 1 (FABP1), and Rho family GTPase 3 (RND3) were reduced in CD tissues. Therefore, SLC39A5, CYP2S1, FA2H, FABP1, and RND3 may be novel biomarkers for CD and associated with severity. Highlights SLC39A5 expression was reduced in CD tissues. Low SLC39A5 expression was associated with CD-simplifying endoscopic score and stenosis. CYP2S1, FA2H, FABP1, and RND3 were reduced in CD tissues. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2689-5307 26895293 |
| Relation: | https://doaj.org/toc/2689-5307 |
| DOI: | 10.1080/26895293.2023.2251705 |
| URL الوصول: | https://doaj.org/article/cc097f3e048045cba9bcc2c450487def |
| رقم الأكسشن: | edsdoj.097f3e048045cba9bcc2c450487def |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 26895307 26895293 |
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| DOI: | 10.1080/26895293.2023.2251705 |