دورية أكاديمية
Single-Cell Assessment of Human Stem Cell-Derived Mesolimbic Models and Their Responses to Substances of Abuse
| العنوان: | Single-Cell Assessment of Human Stem Cell-Derived Mesolimbic Models and Their Responses to Substances of Abuse |
|---|---|
| المؤلفون: | Thomas P. Rudibaugh, Ryan W. Tam, R. Chris Estridge, Samantha R. Stuppy, Albert J. Keung |
| المصدر: | Organoids, Vol 3, Iss 2, Pp 126-147 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | substances of abuse, stem cell, single cell, mesolimbic, organoid, Cytology, QH573-671 |
| الوصف: | The mesolimbic pathway connects ventral tegmental area dopaminergic neurons and striatal medium spiny neurons, playing a critical role in reward and stress behaviors. Exposure to substances of abuse during development and adulthood has been linked to adverse outcomes and molecular changes. The rise of human cell repositories and whole-genome sequences enables human functional genomics ‘in a dish’, offering insights into human-specific responses to substances of abuse. Continued development of new models is needed, and the characterization of in vitro models is also necessary to ensure appropriate experimental designs and the accurate interpretation of results. This study introduces new culture conditions for generating medium spiny neurons and dopaminergic neurons with an early common media, allowing for coculture and assembloid generation. It then provides a comprehensive characterization of these and prior models and their responses to substances of abuse. Single-cell analysis reveals cell-type-specific transcriptomic responses to dopamine, cocaine, and morphine, including compound and cell-type-specific transcriptomic signatures related to neuroinflammation and alterations in signaling pathways. These findings offer a resource for future genomics studies leveraging human stem cell-derived models. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2674-1172 49448765 |
| Relation: | https://www.mdpi.com/2674-1172/3/2/9; https://doaj.org/toc/2674-1172 |
| DOI: | 10.3390/organoids3020009 |
| URL الوصول: | https://doaj.org/article/099b494487654b8d83b08870e1cbd2f1 |
| رقم الأكسشن: | edsdoj.099b494487654b8d83b08870e1cbd2f1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26741172 49448765 |
|---|---|
| DOI: | 10.3390/organoids3020009 |