دورية أكاديمية
Integrated meta-omics reveals the regulatory landscape involved in lipid metabolism between pig breeds
العنوان: | Integrated meta-omics reveals the regulatory landscape involved in lipid metabolism between pig breeds |
---|---|
المؤلفون: | Jiajie Sun, Fang Xie, Jing Wang, Junyi Luo, Ting Chen, Qingyan Jiang, Qianyun Xi, George E. Liu, Yongliang Zhang |
المصدر: | Microbiome, Vol 12, Iss 1, Pp 1-24 (2024) |
بيانات النشر: | BMC, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Microbial ecology |
مصطلحات موضوعية: | Animal model, Intermuscular fat, Single-cell RNA sequencing, Lipidomics, Metagenome, Microbial ecology, QR100-130 |
الوصف: | Abstract Background Domesticated pigs serve as an ideal animal model for biomedical research and also provide the majority of meat for human consumption in China. Porcine intramuscular fat content associates with human health and diseases and is essential in pork quality. The molecular mechanisms controlling lipid metabolism and intramuscular fat accretion across tissues in pigs, and how these changes in response to pig breeds, remain largely unknown. Results We surveyed the tissue-resident cell types of the porcine jejunum, colon, liver, and longissimus dorsi muscle between Lantang and Landrace breeds by single-cell RNA sequencing. Combining lipidomics and metagenomics approaches, we also characterized gene signatures and determined key discriminating markers of lipid digestibility, absorption, conversion, and deposition across tissues in two pig breeds. In Landrace, lean-meat swine mainly exhibited breed-specific advantages in lipid absorption and oxidation for energy supply in small and large intestinal epitheliums, nascent high-density lipoprotein synthesis for reverse cholesterol transport in enterocytes and hepatocytes, bile acid formation, and secretion for fat emulsification in hepatocytes, as well as intestinal-microbiota gene expression involved in lipid accumulation product. In Lantang, obese-meat swine showed a higher synthesis capacity of chylomicrons responsible for high serum triacylglycerol levels in small intestinal epitheliums, the predominant characteristics of lipid absorption in muscle tissue, and greater intramuscular adipcytogenesis potentials from muscular fibro-adipogenic progenitor subpopulation. Conclusions The findings enhanced our understanding of the cellular biology of lipid metabolism and opened new avenues to improve animal production and human diseases. Video Abstract |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2049-2618 |
Relation: | https://doaj.org/toc/2049-2618 |
DOI: | 10.1186/s40168-023-01743-3 |
URL الوصول: | https://doaj.org/article/0df981180fca4c3281882e806013a7dc |
رقم الأكسشن: | edsdoj.0df981180fca4c3281882e806013a7dc |
قاعدة البيانات: | Directory of Open Access Journals |
كن أول من يترك تعليقا!