دورية أكاديمية
Long-term follow-up and exploration of the mechanism of stromal vascular fraction gel in chronic wounds
| العنوان: | Long-term follow-up and exploration of the mechanism of stromal vascular fraction gel in chronic wounds |
|---|---|
| المؤلفون: | Yuan Cai, Fang Zhang, Jingwei Feng, Bihua Wu, Hai Li, Shune Xiao, Feng Lu, Zairong Wei, Chengliang Deng |
| المصدر: | Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-11 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Wound healing, Stromal vascular fraction gel, Adipose stem cells, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Chronic refractory wounds easily relapse and seriously affect the patients’ quality of life. Previous studies have shown that stromal vascular fraction gel (SVF-gel) significantly promotes the early healing of chronic wounds; however, the mechanisms of SVF-gel function per se remain unclear, and a long-term follow-up is lacking. This study aims to explore the mechanisms of SVF-gel promoting the healing of chronic wounds and follow up the long-term efficacy of SVF-gel. Methods Autologous SVF-gel transplantation was performed in 20 patients with chronic wounds (from March 2016 to September 2019), and the size of the wound before and after SVF-gel transplantation was observed. The conditioned medium (CM) was harvested from SVF-gel under serum-free, serum-deprivation and 10% fetal bovine serum (FBS) microenvironment in vitro, respectively. The concentration of the growth factors in the two kinds of gel-CM was tested, and their effects on the proliferation and migration of human dermal fibroblasts (HDFs) were detected. Results All patients had 100% wound closure eventually, and the average time to complete closure was 28.3 ± 9.7 days. The time of follow-up ranged from 2 to 6 years, and there was no wound recurrence. Interestingly, the concentrations of epidermal growth factor and transforming growth factor β1 of the CM were higher in serum-free and serum-deprivation condition than in 10% FBS microenvironment (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-023-03389-2 |
| URL الوصول: | https://doaj.org/article/a0edde02f67444f2a0d1ae8b52a4d09c |
| رقم الأكسشن: | edsdoj.0edde02f67444f2a0d1ae8b52a4d09c |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17576512 |
|---|---|
| DOI: | 10.1186/s13287-023-03389-2 |